Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs.

Autor:	Ashaolu JO; International Health Programme, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan, ROC., Tsai YJ; Taipei City Animal Protection Office -109, Wu-Xin Street, Taipei 11048, Taiwan, ROC., Liu CC; Department of Microbiology, Soochow University, Taipei 111, Taiwan, ROC., Ji DD; International Health Programme, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan, ROC.; Department of Tropical Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan, ROC.
Jazyk:	angličtina
Zdroj:	One health (Amsterdam, Netherlands) [One Health] 2022 Aug 29; Vol. 15, pp. 100430. Date of Electronic Publication: 2022 Aug 29 (Print Publication: 2022).
DOI:	10.1016/j.onehlt.2022.100430
Abstrakt:	Background: It is probable that humans can acquire H. pylori and non- H. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study. Materials and Methods: A total of 95 canine faecal samples were collected from different animal shelters and hospitals in Taipei city. Total DNA was extracted for semi-nested PCR detection of Helicobacter species. The PCR products were sequenced for further comparative database and phylogenetic analyses. Results: The overall prevalence of Helicobacter species in canines of Taipei city was 75.79% (72/95). Two gastric, seven enterohepatic and two unclassified Helicobacter species were identified, all of which have been implicated in the aetiology of human diseases. The predominant species detected included H. canis (27.78%) , H. pylori (26.39%), H. canicola (18.06%) , and H. bilis (13.89%) in decreasing order , while H. canadensis and H. typhlonius were identified for the first time in canines. The genotypes in H. pylori and H. canicola clusters grouped together, with their respective reference strains, showed a close evolutionary distance in the phylogenetic tree, indicating a common ancestry may have existed in these clusters respectively. The residential region of canines, dog living status (pet or stray) and breed (purebred or mixed-breed) are the risk factors associated with Helicobacter infections in the canines examined. Conclusion: The high prevalence of Helicobacter infections in canines highlights a potential public health risk of zoonotic transmission among dogs, humans and other animals, and therefore, the need for proper methods in controlling the transmission routes. In addition, the 16S rRNA gene amplification method was found to be useful for bacterial identification and phylogenetic analysis. (© 2022 The Authors.)
Databáze:	MEDLINE
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