Effect of Dipyridamole on Membrane Energization and Energy Transfer in Chromatophores of Rba. sphaeroides.

Autor: Knox PP; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. knox@biophys.msu.ru., Lukashev EP; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia., Korvatovskii BN; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia., Seifullina NK; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia., Goryachev SN; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia., Allakhverdiev ES; Russian National Medical Research Center of Cardiology, Moscow, 121552, Russia., Paschenko VZ; Faculty of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Jazyk: angličtina
Zdroj: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2022 Oct; Vol. 87 (10), pp. 1138-1148.
DOI: 10.1134/S0006297922100078
Abstrakt: Effect of dipyridamole (DIP) at concentrations up to 1 mM on fluorescent characteristics of light-harvesting complexes LH2 and LH1, as well as on conditions of photosynthetic electron transport chain in the bacterial chromatophores of Rba. sphaeroides was investigated. DIP was found to affect efficiency of energy transfer from the light-harvesting complex LH2 to the LH1-reaction center core complex and to produce the long-wavelength ("red") shift of the absorption band of light-harvesting bacteriochlorophyll molecules in the IR spectral region at 840-900 nm. This shift is associated with the membrane transition to the energized state. It was shown that DIP is able to reduce the photooxidized bacteriochlorophyll of the reaction center, which accelerated electron flow along the electron transport chain, thereby stimulating generation of the transmembrane potential on the chromatophore membrane. The results are important for clarifying possible mechanisms of DIP influence on the activity of membrane-bound functional proteins. In particular, they might be significant for interpreting numerous therapeutic effects of DIP.
Databáze: MEDLINE
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