Study of antibiotic resistance in freshwater ecosystems with low anthropogenic impact.

Autor: Navarro A; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Sanseverino I; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Cappelli F; Department of Science and High Technology, University of Insubria, Via Valleggio 11, 22100 Como, Italy; Water Research Institute IRSA-CNR, Via del Mulino 19, Brugherio 20861, MB, Italy., Lahm A; Bioinformatic project support, P.za S.M. Liberatrice 18, 00153 Roma, Italy., Niegowska M; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Fabbri M; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Paracchini V; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Petrillo M; Seidor Italy srl, Milano, Italy., Skejo H; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy., Valsecchi S; Water Research Institute IRSA-CNR, Via del Mulino 19, Brugherio 20861, MB, Italy., Pedraccini R; Alfa S.r.l., via Carrobbio, 3, 21100 Varese, Italy., Guglielmetti S; Alfa S.r.l., via Carrobbio, 3, 21100 Varese, Italy., Frattini S; Alfa S.r.l., via Carrobbio, 3, 21100 Varese, Italy., Villani G; Energy and Sustainable Economic Development (ENEA), Via E. Fermi 2749, 21027 Ispra, VA, Italy., Lettieri T; European Commission Joint Research Centre, Via E. Fermi 2749, 21027 Ispra, VA, Italy. Electronic address: teresa.lettieri@ec.europa.eu.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Jan 20; Vol. 857 (Pt 3), pp. 159378. Date of Electronic Publication: 2022 Oct 20.
DOI: 10.1016/j.scitotenv.2022.159378
Abstrakt: This study aimed to investigate the bacterial diversity and the background level of antibiotic resistance in two freshwater ecosystems with low anthropogenic impact in order to evaluate the presence of natural antimicrobial resistance in these areas and its potential to spread downstream. Water samples from a pre-Alpine and an Apennine river (Variola and Tiber, respectively) were collected in three different sampling campaigns and bacterial diversity was assessed by 16S sequencing, while the presence of bacteria resistant to five antibiotics was screened using a culturable approach. Overall bacterial load was higher in the Tiber River compared with the Variola River. Furthermore, the study revealed the presence of resistant bacteria, especially the Tiber River showed, for each sampling, the presence of resistance to all antibiotics tested, while for the Variola River, the detected resistance was variable, comprising two or more antibiotics. Screening of two resistance genes on a total of one hundred eighteen bacterial isolates from the two rivers showed that bla TEM , conferring resistance to β-lactam antibiotics, was dominant and present in ~58 % of isolates compared to only ~9 % for mefA/E conferring resistance to macrolides. Moreover, β-lactam resistance was detected in various isolates showing also resistance to additional antibiotics such as macrolides, aminoglycosides and tetracyclines. These observations would suggest the presence of co-resistant bacteria even in non-anthropogenic environments and this resistance may spread from the environment to humans and/or animals.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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