Discovery and Structure-Activity Relationship Studies of Novel Adenosine A 1 Receptor-Selective Agonists.

Autor:	Preti B; Institute of Biochemistry and Molecular Medicine, University of Bern, Bühlstrasse 28, 3012Bern, Switzerland., Suchankova A; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Deganutti G; Centre for Sport, Exercise and Life Sciences, Faculty of Health and Life Sciences, Coventry University, CoventryCV1 5FB, U.K., Leuenberger M; Institute of Biochemistry and Molecular Medicine, University of Bern, Bühlstrasse 28, 3012Bern, Switzerland., Barkan K; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Manulak I; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Huang X; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Carvalho S; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Ladds G; Department of Pharmacology, University of Cambridge, Tennis Court Road, CambridgeCB2 1PD, U.K., Lochner M; Institute of Biochemistry and Molecular Medicine, University of Bern, Bühlstrasse 28, 3012Bern, Switzerland.
Jazyk:	angličtina
Zdroj:	Journal of medicinal chemistry [J Med Chem] 2022 Nov 10; Vol. 65 (21), pp. 14864-14890. Date of Electronic Publication: 2022 Oct 21.
DOI:	10.1021/acs.jmedchem.2c01414
Abstrakt:	A series of benzyloxy and phenoxy derivatives of the adenosine receptor agonists N 6 -cyclopentyl adenosine (CPA) and N 6 -cyclopentyl 5'- N -ethylcarboxamidoadenosine (CP-NECA) were synthesized, and their potency and selectivity were assessed. We observed that the most potent were the compounds with a halogen in the meta position on the aromatic ring of the benzyloxy- or phenoxycyclopentyl substituent. In general, the NECA-based compounds displayed greater A 1 R selectivity than the adenosine-based compounds, with N 6 -2-(3-bromobenzyloxy)cyclopentyl-NECA and N 6 -2-(3-methoxyphenoxy)cyclopentyl-NECA showing ∼1500-fold improved A 1 R selectivity compared to NECA. In addition, we quantified the compounds' affinity and kinetics of binding at both human and rat A 1 R using a NanoBRET binding assay and found that the halogen substituent in the benzyloxy- or phenoxycyclopentyl moiety seems to confer high affinity for the A 1 R. Molecular modeling studies suggested a hydrophobic subpocket as contributing to the A 1 R selectivity displayed. We believe that the identified selective potent A 1 R agonists are valuable tool compounds for adenosine receptor research.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu