De novo transcriptome assembly of Conium maculatum L. to identify candidate genes for coniine biosynthesis.

Autor: Peddinti G; VTT Technical Research Centre of Finland Ltd, Tietotie 2, VTT, P.O. Box 1000, 02044, Espoo, Finland., Hotti H; VTT Technical Research Centre of Finland Ltd, Tietotie 2, VTT, P.O. Box 1000, 02044, Espoo, Finland.; Faculty of Biological and Environmental Sciences, University of Helsinki, PO Box 56, 00014, Helsinki, Finland., Teeri TH; Viikki Plant Science Centre, Department of Agricultural Sciences, University of Helsinki, PO Box 27, 00014, Helsinki, Finland., Rischer H; VTT Technical Research Centre of Finland Ltd, Tietotie 2, VTT, P.O. Box 1000, 02044, Espoo, Finland. heiko.rischer@vtt.fi.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Oct 20; Vol. 12 (1), pp. 17562. Date of Electronic Publication: 2022 Oct 20.
DOI: 10.1038/s41598-022-21728-w
Abstrakt: Poison hemlock (Conium maculatum L.) is a notorious weed containing the potent alkaloid coniine. Only some of the enzymes in the coniine biosynthesis have so far been characterized. Here, we utilize the next-generation RNA sequencing approach to report the first-ever transcriptome sequencing of five organs of poison hemlock: developing fruit, flower, root, leaf, and stem. Using a de novo assembly approach, we derived a transcriptome assembly containing 123,240 transcripts. The assembly is deemed high quality, representing over 88% of the near-universal ortholog genes of the Eudicots clade. Nearly 80% of the transcripts were functionally annotated using a combination of three approaches. The current study focuses on describing the coniine pathway by identifying in silico transcript candidates for polyketide reductase, L-alanine:5-keto-octanal aminotransferase, γ-coniceine reductase, and S-adenosyl-L-methionine:coniine methyltransferase. In vitro testing will be needed to confirm the assigned functions of the selected candidates.
(© 2022. The Author(s).)
Databáze: MEDLINE
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