MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis .

Autor:	de Luxán-Hernández C; Institute of Plant Sciences and Microbiology, University of Hamburg, Hamburg, Germany., Lohmann J; Institute of Plant Sciences and Microbiology, University of Hamburg, Hamburg, Germany., Tranque E; Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas 'Margarita Salas' (CSIC), Madrid, Spain., Chumova J; Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic., Binarova P; Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic., Salinas J; Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas 'Margarita Salas' (CSIC), Madrid, Spain., Weingartner M; Institute of Plant Sciences and Microbiology, University of Hamburg, Hamburg, Germany Magdalena.weingartner@uni-hamburg.de.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2022 Oct 20; Vol. 6 (1). Date of Electronic Publication: 2022 Oct 20 (Print Publication: 2023).
DOI:	10.26508/lsa.202201507
Abstrakt:	The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment supporting its role in coordinating stress response with cell division. Our analysis of a dephosphomutant of MDF suggested how its protein activity might be controlled. Our work uncovers the conserved function of a plant splicing factor and provides novel insight into the interplay of pre-mRNA processing and genome stability in plants. (© 2022 de Luxán-Hernández et al.)
Databáze:	MEDLINE
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