Resveratrol increases tear production and ocular pain after corneal abrasion in male, but not female, rats using a photorefractive keratectomy model.

Autor: Hegarty DM; Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, Portland, OR, 97239, USA., Carroll JR; Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, Portland, OR, 97239, USA., Nguyen D; Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, Portland, OR, 97239, USA., Halls VS; Medicinal Chemistry Core, Oregon Health & Science University, Portland, OR, 97239, USA., Robbins DI; Ted's Brain Science, Inc., Dallas, TX, 75252, USA., Price TJ; Ted's Brain Science, Inc., Dallas, TX, 75252, USA; School of Behavioral and Brain Sciences, Department of Neuroscience, Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, 75080, USA., Dussor G; Ted's Brain Science, Inc., Dallas, TX, 75252, USA; School of Behavioral and Brain Sciences, Department of Neuroscience, Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, 75080, USA., Aicher SA; Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, Portland, OR, 97239, USA. Electronic address: aichers@ohsu.edu.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2022 Dec; Vol. 225, pp. 109281. Date of Electronic Publication: 2022 Oct 18.
DOI: 10.1016/j.exer.2022.109281
Abstrakt: Photorefractive keratectomy (PRK) is an alternative to LASIK and can cause intense acute pain that is often not relieved by standard treatments. To assess potential therapeutics for this type of acute pain, appropriate preclinical models are needed. We describe a preclinical corneal abrasion rat model that simulates the initial stages of PRK surgery and demonstrates similar pain and tear dysfunction as seen clinically. We used both behavioral and homeostatic assays to determine the therapeutic potential of resveratrol on pain and tear production. Studies were conducted in male and female Sprague-Dawley rats. Heptanol was applied to one eye and the superficial corneal epithelium was removed, mimicking the abrasion used in PRK. Spontaneous pain was assessed with orbital tightening (OT) scores for 7 days. Topical resveratrol increased OT scores sex-specifically in abraded males, but not females, at 72 h and 1 week after abrasion. Resveratrol increased tear production in abraded males, with no effect in abraded females. There was no correlation between OT score at 1 week and tear production measurements, demonstrating no relationship between spontaneous ocular pain and tear dysfunction in this model. These findings demonstrate the usefulness of our corneal abrasion preclinical PRK model for the assessment of ocular pain therapeutics and indicate that topical resveratrol may not be useful for managing PRK-induced pain.
Competing Interests: Declaration of competing interest D.M. Hegarty, None; J.R. Carroll, None; D. Nguyen, None; V.S. Halls, None; D.I. Robbins, Ted's Brain Science (O); T.J. Price, Ted's Brain Science (O); G. Dussor, Ted's Brain Science (O); S.A. Aicher, None. Ted's Brain Science is a company developing resveratrol-based therapeutics for pain.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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