P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection.

Autor: Barral TD; Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State 40.110-100, Brazil. Electronic address: tadbarral@gmail.com., Infantes-Lorenzo JA; Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. Electronic address: joseinfa@ucm.es., Moreno I; Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. Electronic address: imoreno@isciii.es., de Garnica García MG; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain. Electronic address: mgarng00@estudiantes.unileon.es., de Val BP; Centre de Recerca en Sanitat Animal (CReSA), IRTA-UAB, 08193 Bellaterra, Barcelona, Spain. Electronic address: bernat.perez@irta.cat., Gortázar C; SaBio-IREC (CSIC-UCLM), 13071 Ciudad Real, Spain. Electronic address: christian.gortazar@uclm.es., Meyer R; Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State 40.110-100, Brazil. Electronic address: rmeyer@ufba.br., Portela RD; Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia State 40.110-100, Brazil. Electronic address: rwportela@ufba.br., Domínguez L; Centro de Vigilancia Sanitaria Veterinaria of the VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, 28040 Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic address: lucasdo@ucm.esg., Domínguez M; Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. Electronic address: mdominguez@isciii.es., Balseiro A; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain; Departamento de Sanidad Animal, Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, 24346 Grulleros, León, Spain. Electronic address: abalm@unileon.es.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2022 Nov-Dec; Vol. 90-91, pp. 101891. Date of Electronic Publication: 2022 Oct 07.
DOI: 10.1016/j.cimid.2022.101891
Abstrakt: The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: