Laticifer growth pattern is guided by cytoskeleton organization.

Autor:	Medina MC; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil., Sousa-Baena MS; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil., Van Sluys MA; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil., Demarco D; Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Oct 03; Vol. 13, pp. 971235. Date of Electronic Publication: 2022 Oct 03 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.971235
Abstrakt:	Laticifers are secretory structures that produce latex, forming a specialized defense system against herbivory. Studies using anatomical approaches to investigate laticifer growth patterns have described their origin; however, their mode of growth, i.e., whether growth is intrusive or diffuse, remains unclear. Studies investigating how cytoskeleton filaments may influence laticifer shape establishment and growth patterns are lacking. In this study, we combined microtubule immunostaining and developmental anatomy to investigate the growth patterns in different types of laticifers. Standard anatomical methods were used to study laticifer development. Microtubules were labelled through immunolocalization of α-tubulin in three types of laticifers from three different plant species: nonanastomosing ( Urvillea ulmacea ), anastomosing unbranched with partial degradation of terminal cell walls ( Ipomoea nil ), and anastomosing branched laticifers with early and complete degradation of terminal cell walls ( Asclepias curassavica ). In both nonanastomosing and anastomosing laticifers, as well as in differentiating meristematic cells, parenchyma cells and idioblasts, microtubules were perpendicularly aligned to the cell growth axis. The analyses of laticifer microtubule orientation revealed an arrangement that corresponds to those cells that grow diffusely within the plant body. Nonanastomosing and anastomosing laticifers, branched or not, have a pattern which indicates diffuse growth. This innovative study on secretory structures represents a major advance in the knowledge of laticifers and their growth mode. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.The reviewer ST declared a shared affiliation with the authors to the handling editor at the time of review. (Copyright © 2022 Medina, Sousa-Baena, Van Sluys and Demarco.)
Databáze:	MEDLINE
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