Knema retusa is antibacterial and antibiofilm against antibiotic resistant Staphylococcus aureus and S. haemolyticus isolated in bovine mastitis.

Autor: Chuprom J; Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand.; School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, 80160, Nakhon Si Thammarat, Thailand.; School of Languages and General Education, Walailak University, 80160, Thasala, Nakhon Si Thammarat, Thailand., Kidsin K; Animal Health Section, Department of Livestock Development, The Eight Reginal Livestock Office, 84000, Surat Thani, Thailand., Sangkanu S; School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, 80160, Nakhon Si Thammarat, Thailand., Nissapatorn V; School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, 80160, Nakhon Si Thammarat, Thailand., Wiart C; The Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, Kota Kin-abalu, 88400, Sabah, Malaysia., de Lourdes Pereira M; Department of Medical Sciences, CICECO-Aveiro Institute of Materials, University of Aveiro, Aveiro, Portugal., Wongtawan T; Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand.; One Health Research Center, Walailak University, 80160, Nakhon Si Thammarat, Thailand., Daus M; Division of Physical Science and Natural Product Center of Excellent, Faculty of Science, Prince of Songkla University, 80160, Songkhla, Thailand., Sotthibandhu DS; Faculty of Medical Technology, Prince of Songkla University, 90110, Songkhla, Thailand., Tipmanee V; Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, 90110, Songkhla, Thailand., Paul AK; School of Pharmacy and Pharmacology, University of Tasmania, 7001, Hobart, TAS, Australia., Scholfield CN; Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand.; One Health Research Center, Walailak University, 80160, Nakhon Si Thammarat, Thailand., Zulkipli MB; School of Pharmacy, University of Nottingham Malaysia Campus, 43500, Semenyih, Malaysia., Abdullah NH; Natural Product Division, Forest Research Institute Malaysia (FRIM), 52109, Kepong, Malaysia., Mitsuwan W; Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand. watcharapong.mi@wu.ac.th.; One Health Research Center, Walailak University, 80160, Nakhon Si Thammarat, Thailand. watcharapong.mi@wu.ac.th.; Center of Excellence in Innovation of Essential Oil, Walailak University, 80160, Nakhon Si Thammarat, Thailand. watcharapong.mi@wu.ac.th.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2023 Jun; Vol. 47 (2), pp. 523-538. Date of Electronic Publication: 2022 Oct 19.
DOI: 10.1007/s11259-022-09999-0
Abstrakt: This study aimed to assess antibacterial activity of Knema retusa wood extract (KRe) against antibiotic resistant staphylococci which are causative agents of bovine mastitis. From 75 cases of intramammary infections in dairy cows, 66 staphylococcal isolates were collected, including 11 Staphylococcus aureus isolates (17%) and 55 coagulase-negative staphylococci (83%). Sixty isolates (91%) formed strong biofilms. KRe had minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) against the isolates ranging 32-256 ug/mL and 64-512 ug/mL, respectively. Two-hour KRe exposures at 4×MIC, viabilities of S. aureus and S. haemolyticus decreased by 3 log 10 compared to the control. Scanning EM (SEM) showed that KRe disrupted the bacterial cells of both species. KRe at 1/16×MIC significantly inhibited biofilm formation (P < 0.05) in both S. aureus and S. haemolyticus. At 1/2×MIC, S. aureus and S. haemolyticus biofilm inhibition ranged from 75 to 99%. Cells within established biofilms were disrupted 66-83% by KRe at 32×MIC. Moreover, 1/2×MIC KRe reduced bacterial adhesion to glass surfaces observed by SEM. According to GC-MS analysis, the major compound in KRe was endo-2-hydroxy-9,9-(ethylenedioxy)-1-carbethoxy bicyclo [3.3.1] nonane (E2N). Molecular docking analysis of E2N has a high affinity for staphylococcal accessory regulator A (SarA), binding free-energy - 6.40kcal/mol. The results suggested that KRe may have medicinal benefits by inhibiting the growth, biofilm, and adhesion of antibiotic resistant staphylococci isolated from bovine mastitis.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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