The function of chitosan/agarose biopolymer on Fe 2 O 3 nanoparticles and evaluation of their effects on MCF-7 breast cancer cell line and expression of BCL2 and BAX genes.

Autor: Nouri Hajbaba M; Department of biology, Faculty of Science, NourDanesh Institute of Higher Education, Isfahan, Iran., Pourmadadi M; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran., Yazdian F; Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran., Rashedi H; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran., Abdouss M; Department of Chemistry, Amirkabir University of Technology, Tehran, Iran., Zhohrabi DS; Department of biology, Faculty of Science, NourDanesh Institute of Higher Education, Isfahan, Iran.
Jazyk: angličtina
Zdroj: Biotechnology progress [Biotechnol Prog] 2023 Jan; Vol. 39 (1), pp. e3305. Date of Electronic Publication: 2022 Nov 01.
DOI: 10.1002/btpr.3305
Abstrakt: In recent decades, magnetic nanoparticles modified with biocompatible polymers have been recognized as a suitable tool for treating breast cancer. The aim of this research was to evaluate the function of chitosan/agarose-functionalized Fe 2 O 3 nanoparticles on the MCF-7 breast cancer cell line and the expression of BCL2 and BAX genes. Free Fe 2 O 3 nanoparticles were prepared by hydrothermal method. FTIR, XRD, SEM, DLS, VSM, and zeta potential analyses determined the size and morphological characteristics of the synthesized nanoparticles. The effect of Fe 2 O 3 free nanoparticles and formulated Fe 2 O 3 nanoparticles on induction of apoptosis was studied by double-dye Annexin V-FITC and PI. Also, the gene expression results using the PCR method displayed that Fe 2 O 3 formulated nanoparticles induced BAX apoptosis by increasing the anti-apoptotic gene expression and decreasing the expression of pro-apoptotic gene BCL2, so the cell progresses to planned cell death. In addition, the results showed that the BAX/BCL2 ratio decreased significantly after treatment of MCF-7 cells with free Fe 2 O 3 nanoparticles, and the BAX/BCL2 ratio for Fe 2 O 3 formulated nanoparticles increased significantly. Also, to evaluate cell migration, the scratch test was performed, which showed a decrease in motility of MCF-7 cancer cells treated with Fe 2 O 3 nanoparticles formulated with chitosan/agarose at concentrations of 10, 50, 100, and 200 μg/ml.
(© 2022 American Institute of Chemical Engineers.)
Databáze: MEDLINE
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