Tissue integration and biodegradation of soft tissue substitutes with and without compression: an experimental study in the rat.

Autor: Bienz SP; Clinic of Reconstructive Dentistry, University of Zurich, Zurich, Switzerland. stefan.bienz@zzm.uzh.ch., Vaquette C; School of Dentistry, The University of Queensland, Herston, QLD, Australia., Ioannidis A; Clinic of Reconstructive Dentistry, University of Zurich, Zurich, Switzerland., Hämmerle CHF; Clinic of Reconstructive Dentistry, University of Zurich, Zurich, Switzerland., Jung RE; Clinic of Reconstructive Dentistry, University of Zurich, Zurich, Switzerland., Ivanovski S; School of Dentistry, The University of Queensland, Herston, QLD, Australia., Thoma DS; Clinic of Reconstructive Dentistry, University of Zurich, Zurich, Switzerland.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2023 Jan; Vol. 27 (1), pp. 313-328. Date of Electronic Publication: 2022 Oct 18.
DOI: 10.1007/s00784-022-04726-0
Abstrakt: Objectives: To analyze the influence of compression on tissue integration and degradation of soft tissue substitutes.
Material and Methods: Six subcutaneous pouches in twenty-eight rats were prepared and boxes made of Al 2 O 3 were implanted and used as carriers for soft tissue substitutes: a collagen matrix (MG), two volume-stable collagen matrices (FG/MGA), and a polycaprolactone scaffold(E). The volume-stable materials (FG/MGA/E) were further implanted with a twofold (2) and a fourfold (4) compression, created by the stacking of additional layers of the substitute materials. The samples were retrieved at 1, 2, and 12 weeks (10 groups, 3 time points, n = 5 per time point and group, overall, 150 samples). The area fraction of infiltrated fibroblasts and inflammatory cells was evaluated histologically. Due to within-subject comparisons, mixed models were conducted for the primary outcome. The level of significance was set at 5%.
Results: The area fraction of fibroblasts increased in all groups over time. At 12 weeks, the densely compressed materials FG4 (1.1%), MGA4 (1.7%), and MGA2 (2.5%) obtained lower values as compared to the other groups, ranging between 4.7 (E2) and 6.5% (MG). Statistically significant differences (p ≤ 0.05) were observed between groups FG4 vs MG/FG2/E/E4 as well as between MGA4 vs MG/FG2/E/E4 and E vs MGA2.
Conclusions: Higher levels of compression led to delayed tissue integration. The effect of different compression levels was more distinct when compared to the differences between the materials.
Clinical Relevance: All biomaterials demonstrated tissue integration and a minimal concomitant inflammatory reaction. Clinically, it might be more favorable to obtain a sufficient flap release or to reduce the material size to improve the tissue integration processes.
(© 2022. The Author(s).)
Databáze: MEDLINE
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