| Autor: |
Mmatli M; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa., Leshaba TMS; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa., Skosana LB; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa.; Tshwane Academic Division, Department of Medical Microbiology, National Health Laboratory Service, Pretoria, South Africa., Mbelle NM; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa., Osei Sekyere J; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa.; Department of Dermatology, School of Medicine, University of Pretoria, Pretoria, South Africa. |
| Jazyk: |
angličtina |
| Zdroj: |
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2022 Nov; Vol. 28 (11), pp. 1028-1036. Date of Electronic Publication: 2022 Oct 12. |
| DOI: |
10.1089/mdr.2022.0112 |
| Abstrakt: |
Background: Extensive use of carbapenems to treat multidrug-resistant (MDR) Gram-negative bacteria (GNB) facilitates the wide dissemination of carbapenemase-producing carbapenem-resistant GNB. Colistin was reintroduced into clinical settings to manage these GNB infections. However, there is currently an increase in the dissemination of mobile colistin resistance ( mcr) -producing colistin-resistant GNB isolates in clinical settings. The epidemiology of carbapenemases and mcr in Pretoria was evaluated. Methods: Clinical MDR GNB were collected and screened for carbapenemases and mcr using polymerase chain reaction (PCR); their antibiotic susceptibility profiles were elucidated using the Vitek ® 2 automated system (Biomerieux, France) and microbroth dilution (for colistin). Results and Discussion: A total of 306 isolates were collected; a majority of these were Klebsiella pneumoniae ( n = 208) and were collected from males ( n = 158). The isolates were retrieved from a variety of infection sites, including urine, blood cultures, and rectal swabs. The Vitek 2 system found that these isolates were largely resistant to β-lactams, where 217 (70.9%) had reduced susceptibility to at least one carbapenem (ertapenem, meropenem, or imipenem), and 81 isolates (26.5%) were resistant to colistin. PCR screening identified 201 (65.7%) isolates harboring carbapenemase genes consisting of bla OXA-48 (170, 84.2%), bla NDM (31, 15.4%), bla IMP (5, 2%), bla KPC (4, 1%), and bla VIM (5, 2%). Furthermore, 14 bla OXA-48 -producing isolates were coharboring bla VIM (2), bla NDM (9), bla KPC (1), and bla IMP (2) genes. Only one isolate harbored the mobile colistin resistance ( mcr ) -1 gene, and this is the first report of an mcr-1 -producing Acinetobacter baumannii isolate in South Africa. Conclusion: There is high endemicity of carbapenemase genes and a low prevalence of mcr genes in GNB, particularly in K. pneumoniae, in health care facilities in Pretoria and surrounding regions of South Africa. Significance: Health care facilities in Pretoria are becoming breeding grounds for MDR infections that threaten public health. Careful use of carbapenems and other antibiotics is necessary to prevent further escalation and outbreak of these MDR strains that can claim several lives. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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