Amaranth calcium oxalate crystals are associated with chloroplast structures and proteins.

Autor: Cerritos-Castro IT; Molecular Biology Division, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico., Patrón-Soberano A; Molecular Biology Division, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico., Bojórquez-Velázquez E; Molecular Biology Division, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico., González-Escobar JL; Molecular Biology Division, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico., Vargas-Ortiz E; Facultad de Agrobiología, Universidad Michoacana de San Nicolás de Hidalgo, Uruapan, Mexico., Muñoz-Sandoval E; Advanced Materials, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico., Barba de la Rosa AP; Molecular Biology Division, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., San Luis Potosí, Mexico.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2022 Nov; Vol. 85 (11), pp. 3694-3706. Date of Electronic Publication: 2022 Aug 17.
DOI: 10.1002/jemt.24221
Abstrakt: Calcium oxalate (CaOx) crystals in plants are formed in crystal idioblasts cells and have specific geometric shapes. Their proposed functions include calcium homeostasis and carbon source, among others. Amaranth is a plant that presents high tolerance to abiotic stresses and accumulates considerable amounts of CaOx crystals; however, few studies have focused on characterizing the crystals ultrastructure and none is related to identifying proteins bound to them. This information is of great interest to understand the mechanisms related to CaOx crystal formation and to support their proposed functions. Thus, this work aimed to characterize CaOx crystals in amaranth leaves. Crystals were purified and the proteins bound to them were isolated and identified by nLC-MS/MS. Leaf sections were analyzed by light and electron microscopy. The identified proteins were related to the chloroplast such as ATPb synthase, RuBisCO large subunit, and cell wall-related proteins, which were validated by immunohistochemistry and immunogold labeling. In addition, it was observed that CaOx crystal idioblasts were formed from parenchyma cells associated with mesophyll and veins, in which the thylakoid membranes of degraded chloroplasts turned into crystal chambers. These results significantly advance our understanding of the mechanisms of CaOx crystal formation and the potential function as an alternative carbon source in leaves.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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