Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts.

Autor:	Charlie-Silva I; Department of Pharmacology, University of São Paulo, São Paulo, Brazil., Feitosa NM; Integrated Laboratory of Translational Bioscience, Institute of Biodiversity and Sustainability, Federal University of Rio de Janeiro, Macaé, Brazil., Pontes LG; Department Immunology, University of São Paulo, São Paulo, Brazil., Fernandes BH; Laboratório de Controle Genético e Sanitário, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil., Nóbrega RH; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University, São Paulo, Brazil., Gomes JMM; Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil., Prata MNL; Department of Pharmacology, Instituto de CiênciasBiomédicas-Universidade Federal de Minas Gerais (ICB-UFMG), Belo Horizonte, Brazil., Ferraris FK; Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil., Melo DC; Laboratory of Zebrafish from Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil., Conde G; Department of Preventive Veterinary Medicine, São Paulo State University, São Paulo, Brazil., Rodrigues LF; Department of Preventive Veterinary Medicine, São Paulo State University, São Paulo, Brazil., Aracati MF; Department of Preventive Veterinary Medicine, São Paulo State University, São Paulo, Brazil., Corrêa-Junior JD; Department of Morphology, Instituto de CiênciasBiomédicas-Universidade Federal de Minas Gerais (ICB-UFMG), Belo Horizonte, Brazil., Manrique WG; Veterinary College, Federal University of Rondonia, Rolim de Moura, Brazil., Superio J; Department of Aquaculture, Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway., Garcez AS; Department of Lasers in Dentistry, São Leopoldo Mandic, Campinas, Brazil., Conceição K; Peptide Biochemistry Laboratory, Universidade Federal de São Paulo (UNIFESP), Sao Jose Dos Campos, Brazil., Yoshimura TM; Center for Lasers and Applications, Instituto de PesquisasEnergéticas e Nucleares (IPEN-CNEN), Sao Paulo, Brazil., Núñez SC; University Brazil, São Paulo, Brazil.; University Brazil, Descalvado, Brazil., Eto SF; Development and Innovation Laboratory, Center of Innovation and Development, Butantan Institute, São Paulo, Brazil., Fernandes DC; Department of Preventive Veterinary Medicine, São Paulo State University, São Paulo, Brazil., Freitas AZ; Center for Lasers and Applications, Instituto de PesquisasEnergéticas e Nucleares (IPEN-CNEN), Sao Paulo, Brazil., Ribeiro MS; Center for Lasers and Applications, Instituto de PesquisasEnergéticas e Nucleares (IPEN-CNEN), Sao Paulo, Brazil., Nedoluzhko A; Paleogenomics Laboratory, European University at Saint Petersburg, Saint Petersburg, Russia., Lopes-Ferreira M; Immunoregulation Unit, Butantan Institute, São Paulo, Brazil., Borra RC; Department of Genetics and Evolution, Federal University of São Carlos, São Paulo, Brazil., Barcellos LJG; Postgraduate Program in Pharmacology, Federal University of Santa Maria, Rio Grande do Sul, Brazil.; Postgraduate Program in Bioexperimentation. University of Passo Fundo, Rio Grande do Sul, Brazil., Perez AC; Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil., Malafaia G; Biological Research Laboratory, Goiano Federal Institute, Urutaí, Brazil., Cunha TM; Center of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil.; Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil., Belo MAA; Department of Preventive Veterinary Medicine, São Paulo State University, São Paulo, Brazil.; University Brazil, São Paulo, Brazil.; University Brazil, Descalvado, Brazil., Galindo-Villegas J; Department of Genomics, Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Sep 29; Vol. 13, pp. 1019201. Date of Electronic Publication: 2022 Sep 29 (Print Publication: 2022).
DOI:	10.3389/fimmu.2022.1019201
Abstrakt:	Regulation of inflammation is a critical process for maintaining physiological homeostasis. The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae ( Chondrus crispus ) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation. However, the associated mechanisms in vertebrates are not well understood. Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) ( Danio rerio ). We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence. Nevertheless, they generate a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity. Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level. Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN. First, the bioinformatic analysis revealed the composition of the plasma proteome. Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group. Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant ( p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated. Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group. Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation. Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response. Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Charlie-Silva, Feitosa, Pontes, Fernandes, Nóbrega, Gomes, Prata, Ferraris, Melo, Conde, Rodrigues, Aracati, Corrêa-Junior, Manrique, Superio, Garcez, Conceição, Yoshimura, Núñez, Eto, Fernandes, Freitas, Ribeiro, Nedoluzhko, Lopes-Ferreira, Borra, Barcellos, Perez, Malafaia, Cunha, Belo and Galindo-Villegas.)
Databáze:	MEDLINE
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