Antibiotic-Resistant Bacteria and Resistance Genes in Isolates from Ghanaian Drinking Water Sources.

Autor: Odonkor ST; School of Public Services and Governance, Ghana Institute of Management and Public Administration, Accra, Ghana., Simpson SV; Noguchi Memorial Institute for Medical Research LG 581, Legon-Accra, Ghana., Morales Medina WR; Microbiology & Molecular Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ, USA., Fahrenfeld NL; Civil & Environmental Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Jazyk: angličtina
Zdroj: Journal of environmental and public health [J Environ Public Health] 2022 Oct 06; Vol. 2022, pp. 2850165. Date of Electronic Publication: 2022 Oct 06 (Print Publication: 2022).
DOI: 10.1155/2022/2850165
Abstrakt: The control of infectious diseases is seriously threatened by the increase in the number of microorganisms resistant to antimicrobial agents. Antibiotic-resistant bacteria have also been identified in the water environment. A field study was performed sampling drinking water sources in seven districts of southern Ghana targeting boreholes, dams, hand-dug wells, and streams during baseflow conditions. Bacteria were isolated ( N = 110) from a total of 67 water samples to investigate their antimicrobial susceptibility and to determine their carriage of select antibiotic resistance genes. Bacterial identification was performed using conventional selective media methods and the analytical profile index (API) method. Antibiotic susceptibility tests were carried out using the Kirby-Bauer method. Results indicated that all water sources tested were of poor quality based on the presence of fecal indicator organisms. The most commonly occurring bacterium isolated from water was Klebsiella spp. ( N = 24, 21.8%), followed by E. coli ( N = 23, 20.9%). Gram-negative bacteria isolates were most commonly resistant to cefuroxime (24.5%), while the Gram-positives were most commonly resistant to meropenem (21.3%). The highest rates of bacterial resistances to more than one antibiotic were observed in Klebsiella spp. (30.0%) followed by E. coli (27.8%). PCR was used to detect the presence of a select antibiotic resistance genes in the Gram-negative isolates. The presence of bla NDM-1 , sul l, tet (O), and tet (W) were observed in isolates from all water sources. In contrast, erm F was not detected in any of the Gram-negative isolates from any water source. Most (28.7%) of the resistance genes were observed in E. coli isolates. Reducing microbial contamination of the various water sources is needed to protect public health and to ensure the sustainability of this resource. This further calls for education of the citizenry.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2022 Stephen T. Odonkor et al.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje