Drug Delivery in Plants Using Silk Microneedles.

Autor: Cao Y; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Koh SS; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore.; Department of Biological Sciences, National University of Singapore, Singapore, 119077, Singapore., Han Y; Singapore-MIT Alliance for Research and Technology, Singapore, 119077, Singapore., Tan JJ; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore., Kim D; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Chua NH; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore.; Singapore-MIT Alliance for Research and Technology, Singapore, 119077, Singapore., Urano D; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, 117604, Singapore.; Department of Biological Sciences, National University of Singapore, Singapore, 119077, Singapore.; Singapore-MIT Alliance for Research and Technology, Singapore, 119077, Singapore., Marelli B; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Singapore-MIT Alliance for Research and Technology, Singapore, 119077, Singapore.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2023 Jan; Vol. 35 (2), pp. e2205794. Date of Electronic Publication: 2022 Dec 08.
DOI: 10.1002/adma.202205794
Abstrakt: New systems for agrochemical delivery in plants will foster precise agricultural practices and provide new tools to study plants and design crop traits, as standard spray methods suffer from elevated loss and limited access to remote plant tissues. Silk-based microneedles can circumvent these limitations by deploying a known amount of payloads directly in plants' deep tissues. However, plant response to microneedles' application and microneedles' efficacy in deploying physiologically relevant biomolecules are unknown. Here, it is shown that gene expression associated with Arabidopsis thaliana wounding response decreases within 24 h post microneedles' application. Additionally, microinjection of gibberellic acid (GA 3 ) in A. thaliana mutant ft-10 provides a more effective and efficient mean than spray to activate GA 3 pathways, accelerating bolting and inhibiting flower formation. Microneedle efficacy in delivering GA 3 is also observed in several monocot and dicot crop species, i.e., tomato (Solanum lycopersicum), lettuce (Lactuca sativa), spinach (Spinacia oleracea), rice (Oryza Sativa), maize (Zea mays), barley (Hordeum vulgare), and soybean (Glycine max). The wide range of plants that can be successfully targeted with microinjectors opens the doors to their use in plant science and agriculture.
(© 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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