The expression of stromal biomarkers in small papillary thyroid carcinomas.

Autor: Bayadsi H; Department of Surgical and Perioperative Sciences/Surgery, Umeå University, Umeå, Sweden. haytham.bayadsi@regionjh.se., Barghout G; Department of Clinical Pathology, Sunderby Hospital, Sunderbyn, Sweden., Gustafsson M; Department of Surgical and Perioperative Sciences/Surgery, Umeå University, Umeå, Sweden., Sund M; Department of Surgical and Perioperative Sciences/Surgery, Umeå University, Umeå, Sweden.; Clinicum/Department of Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Hennings J; Department of Surgical and Perioperative Sciences/Surgery, Umeå University, Umeå, Sweden.
Jazyk: angličtina
Zdroj: World journal of surgical oncology [World J Surg Oncol] 2022 Oct 14; Vol. 20 (1), pp. 340. Date of Electronic Publication: 2022 Oct 14.
DOI: 10.1186/s12957-022-02805-w
Abstrakt: Background: The importance of stroma for tumor progression is recognized for many cancer types. In this study, we aim to evaluate the expression of types I (Col1) and IV (Col4) collagens, alpha-smooth muscle actin (a-SMA), and matrix metallopeptidase 9 (MMP-9) in the tumor stroma of small papillary thyroid carcinoma (PTC).
Material and Methods: Twenty-five non-metastatic small PTCs (pT1N0) and nineteen metastatic small PTCs (pT1N1b) including corresponding metastatic lateral lymph nodes were selected and paraffinized tissue blocks retrieved. The samples were stained for Col1, COL4, a-SMA, and MMP-9 antibodies using immunohistochemistry. The expression of the stromal proteins was scored and analyzed based on the location, intensity, and distribution.
Results: Col1 and Col4 expression were significantly higher in normal thyroid tissue compared to PTC tissue. On the contrary, expression of a-SMA and MMP-9 was higher in PTC tissue compared to normal thyroid tissue. Both Col1 and Col4 were significantly more highly expressed in the non-metastatic tumors compared with metastatic tumors. The expression of a-SMA and MMP9 was slightly, but not significantly, higher in the metastasized tumors and their respective lymph nodes. There was a significant correlation between the metastasized tumors and their respective lymph nodes in Col1 and MMP-9 expression.
Conclusions: Col1, Col4, a-SMA, and MMP-9 expression in PTCs differs significantly from that of normal thyroid tissue. The higher expression of Col1 and Col4 in normal thyroid tissue and in the non-metastasized tumors indicates that Col1 and 4 might have a potential protective role in tumor progression. The higher expression of a-SMA and MMP9 in PTCs indicates that these proteins might have a role in promoting PTC progression and aggressiveness.
(© 2022. The Author(s).)
Databáze: MEDLINE
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