Detecting aquatic pathogens with field-compatible dried qPCR assays.

Autor: Rieder J; Institute for Fish and Wildlife Health, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland. Electronic address: jessica.rieder@unibe.ch., Martin-Sanchez PM; Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern 0316, Oslo, Norway; Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avenida Reina Mercedes 10, 41012 Seville, Spain. Electronic address: p.m.martin-sanchez@ibv.uio.no., Osman OA; eDNA Solutions AB, Bjorkasgatan 16, SE-43131 Molndal, Gothenburg, Sweden. Electronic address: omneya@ednasolutions.se., Adrian-Kalchhauser I; Institute for Fish and Wildlife Health, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland. Electronic address: irene.adrian-kalchhauser@unibe.ch., Eiler A; Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern 0316, Oslo, Norway; eDNA Solutions AB, Bjorkasgatan 16, SE-43131 Molndal, Gothenburg, Sweden. Electronic address: alexander.eiler@ibv.uio.no.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2022 Nov; Vol. 202, pp. 106594. Date of Electronic Publication: 2022 Oct 12.
DOI: 10.1016/j.mimet.2022.106594
Abstrakt: Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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