Phenotypic and genomic characterization provide new insights into adaptation to environmental stressors and biotechnological relevance of mangrove Alcaligenes faecalis D334.

Autor: Quach NT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam., Loan TT; Department of Microbiology, Faculty of Biology, University of Science, Vietnam National University, Hanoi (VNU), Hanoi 100000, Viet Nam; Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, Hanoi 100000, Viet Nam., Nguyen TTA; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam., Nguyen Vu TH; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam., Pham QA; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam., Chu HH; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam., Phi QT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Viet Nam. Electronic address: tienpq@ibt.ac.vn., Thuoc DV; Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, Hanoi 100000, Viet Nam. Electronic address: thuocdv@hnue.edu.vn.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2023 Jan-Feb; Vol. 174 (1-2), pp. 103994. Date of Electronic Publication: 2022 Oct 12.
DOI: 10.1016/j.resmic.2022.103994
Abstrakt: Alcaligenes faecalis D334 was determined in this study as a salt-tolerant bacterium isolated from mangrove sediment. In response to 6% (w/v) NaCl, strain D334 produced the highest ectoines of 14.14 wt%. To understand adaptive features to mangrove environment, strain D334 was sequenced using Pacific BioScience platform, resulting in a circular chromosome of 4.23 Mb. Of note, D334 genome harbored 81 salt-responsive genes, among which two membrane-associated genes ompc and eric were absent in 3 selected A. faecalis genomes. Apart from that, a complete pathway for ectoine and 5-hydroxyectoine synthesis was predicted. To resist 40 mM H 2 O 2 , 46 genetic determinants contributing to oxidative stress response were employed. Moreover, two operons involved in polyhydroxyalkanoate (PHA) production were identified in the D334 genome, resulting in maximum PHA content of 5.03 ± 0.04 wt% and PHA concentration of 0.13 ± 0.001 g/L. A large flagellar biosynthesis operon contributing to swimming motility was found to be conserved in D334 and 8 other A. faecalis genomes. These findings shed light for the first time on the high versatility of A. faecalis D334 genome to adapt to mangrove lifestyle and the possibility to develop D334 as an industrial platform for PHA and 5-hydroxyectoine production.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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