| Autor: |
Cao Z; School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, Anhui, P. R. China.; Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, CAS; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, CAS, Hefei 230031, Anhui, P. R. China., Wang M; Department of Pathophysiology, School of Basic Medical Science, Anhui Medical University, No. 81 Mei-Shan Rd, Hefei 230032, Anhui, P. R. China., Zhou T; Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, CAS; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, CAS, Hefei 230031, Anhui, P. R. China., Xu A; Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, CAS; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, CAS, Hefei 230031, Anhui, P. R. China., Du H; Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, CAS; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, CAS, Hefei 230031, Anhui, P. R. China. |
| Abstrakt: |
Endosulfan is an extensively used organochlorine pesticide around the world, which was classified as a persistent organic pollutant (POP) in 2009. Although previous studies have documented the reproductive toxicity of endosulfan in a variety of organisms, little is known about the influence of endosulfan on the genome stability of germ cells and nonexposed progeny. Here we applied whole-genome sequencing to explore the germ cell mutagenicity of α-endosulfan in Caenorhabditis elegans ( C. elegans ). We found that, although low doses of α-endosulfan exhibited a minor effect on the reproductive capacity of C. elegans , chronic exposure to 1 μM α-endosulfan significantly increased the mutation frequencies of nonexposed progeny. Further analysis of genome-wide mutation spectra demonstrated that α-endosulfan preferentially elicited A:T → G:C substitutions and clustered mutations. By using worms deficient in DNA damage response genes, our results suggest the involvement of translesion synthesis polymerase η in modulating α-endosulfan-induced mutations in germ cells. Together, these observations reveal the germ cell mutagenicity of α-endosulfan in C. elegans and the possible underlying mechanism. In addition, our findings implicate that germ cell mutagenicity might be a necessary consideration for the health risk assessment of environmental chemicals such as POPs. |
