Biosynthesis and Properties of a P(3HB- co -3HV- co -4HV) Produced by Cupriavidus necator B-10646.

Autor: Zhila NO; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia., Sapozhnikova KY; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russia., Kiselev EG; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia., Nemtsev IV; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.; L.V. Kirensky Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/38 Akademgorodok, Krasnoyarsk 660036, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences' 50 Akademgorodok, Krasnoyarsk 660036, Russia., Lukyanenko AV; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.; L.V. Kirensky Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/38 Akademgorodok, Krasnoyarsk 660036, Russia., Shishatskaya EI; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia., Volova TG; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.; Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.
Jazyk: angličtina
Zdroj: Polymers [Polymers (Basel)] 2022 Oct 09; Vol. 14 (19). Date of Electronic Publication: 2022 Oct 09.
DOI: 10.3390/polym14194226
Abstrakt: Synthesis of P(3HB- co -3HV- co -4HV) copolymers by the wild-type strain Cupriavidus necator B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA, DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (C x 38-49%) molecular weight characteristics M n (45-87 kDa), and M w (201-248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB- co -3HV- co -4HV) copolymers using Cupriavidus necator B-10646.
Competing Interests: The authors declare that they have no conflict of interest in the publication of this article. The authors have no conflicts of interest to report in this work. The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript.
Databáze: MEDLINE
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