| Autor: |
García-Cerna S; Laboratorio 7 de la Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Avenida Universidad No. 1001, Chamilpa, Cuernavaca C.P. 62209, Morelos, Mexico., Sánchez-Pacheco U; Laboratorio 7 de la Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Avenida Universidad No. 1001, Chamilpa, Cuernavaca C.P. 62209, Morelos, Mexico., Meneses-Acosta A; Laboratorio 7 de la Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Avenida Universidad No. 1001, Chamilpa, Cuernavaca C.P. 62209, Morelos, Mexico., Rojas-García J; CIATEQ A. C. Plasticos y Materiales Avanzados. Av. Del Retablo 150, Queretaro C.P. 76150, Queretaro, Mexico., Campillo-Illanes B; Instituto de Ciencias Fisicas, Universidad Nacional Autonoma de Mexico, Avenida Universidad S/N, Chamilpa, Cuernavaca C.P. 62210, Morelos, Mexico., Segura-González D; Departamento de Ingenieria Celular y Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo. Post. 510-3, Cuernavaca C.P. 62250, Morelos, Mexico., Peña-Malacara C; Departamento de Ingenieria Celular y Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo. Post. 510-3, Cuernavaca C.P. 62250, Morelos, Mexico. |
| Abstrakt: |
Advances in tissue engineering have made possible the construction of organs and tissues with the use of biomaterials and cells. Three important elements are considered: a specific cell culture, an adequate environment, and a scaffold. The present study aimed to develop P3HB scaffolds by 3D printing and evaluate their biocompatibility with HaCaT epidermal cells, as a potential model that allows the formation of functional tissue. By using a method of extraction and purification with ethanol and acetone, a biopolymer having suitable properties for use as a tissue support was obtained. This polymer exhibited a higher molecular weight (1500 kDa) and lower contact angle (less than 90°) compared to the material obtained using the conventional method. The biocompatibility analysis reveals that the scaffold obtained using the ethanol-acetone method and produced by 3D printing without pores was not cytotoxic, did not self-degrade, and allowed high homogenous cell proliferation of HaCaT cells. In summary, it is possible to conclude that the P3HB scaffold obtained by 3D printing and a simplified extraction method is a suitable support for the homogeneous development of HaCaT keratinocyte cell lineage, which would allow the evaluation of this material to be used as a biomatrix for tissue engineering. |
