Autor: |
Bie BJ; School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China., Zhao XR; School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China., Yan JR; Faculty of Science, University of Melbourne, Grattan Street, Parkville, VIC 3010, Australia., Ke XJ; School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China., Liu F; School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China., Yan GP; School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China. |
Jazyk: |
angličtina |
Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2022 Oct 10; Vol. 27 (19). Date of Electronic Publication: 2022 Oct 10. |
DOI: |
10.3390/molecules27196747 |
Abstrakt: |
Fluorescent imaging has been expanded, as a non-invasive diagnostic modality for cancers, in recent years. Fluorescent probes in the near-infrared window can provide high sensitivity, resolution, and signal-to-noise ratio, without the use of ionizing radiation. Some fluorescent compounds with low molecular weight, such as rhodamine B (RhB) and indocyanine green (ICG), have been used in fluorescent imaging to improve imaging contrast and sensitivity; however, since these probes are excreted from the body quickly, they possess significant restrictions for imaging. To find a potential solution to this, this work investigated the synthesis and properties of novel macromolecular fluorescent compounds. Herein, water-soluble dextran fluorescent compounds (SD-Dextran-RhB) were prepared by the attachment of RhB and sulfadiazine (SD) derivatives to dextran carrier. These fluorescent compounds were then characterized through IR, 1 H NMR, 1 3 C NMR, UV, GPC, and other methods. Assays of their cellular uptake and cell cytotoxicity and fluorescent imaging were also performed. Through this study, it was found that SD-Dextran-RhB is sensitive to acidic conditions and possesses low cell cytotoxicities compared to normal 293 cells and HepG2 and HeLa tumor cells. Moreover, SD-Dextran-RhB demonstrated good fluorescent imaging in HepG2 and HeLa cells. Therefore, SD-Dextran-RhB is suitable to be potentially applied as a probe in the fluorescent imaging of tumors. |
Databáze: |
MEDLINE |
Externí odkaz: |
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|