| Autor: |
Minich A; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia., Lišková V; Biomedical Research Center, Institute of Clinical and Translational Research, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia., Kormanová Ľ; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia., Krahulec J; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia., Šarkanová J; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia., Mikulášová M; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia., Levarski Z; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia.; Science Park, Comenius University in Bratislava, Ilkovičova 8, 841 04 Bratislava, Slovakia., Stuchlík S; Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 841 04 Bratislava, Slovakia.; Science Park, Comenius University in Bratislava, Ilkovičova 8, 841 04 Bratislava, Slovakia. |
| Abstrakt: |
Staphylococcus epidermidis is a known opportunistic pathogen and is one of the leading causes of chronic biofilm-associated infections. Biofilm formation is considered as a main strategy to resist antibiotic treatment and help bacteria escape from the human immune system. Understanding the complex mechanisms in biofilm formation can help find new ways to treat resistant strains and lower the prevalence of nosocomial infections. In order to examine the role of RNAIII regulated by the agr quorum sensing system and to what extent it influences biofilm resistance to antimicrobial agents, deletion mutant S. epidermidis RP62a-ΔRNAIII deficient in repressor domains with a re-maining functional hld gene was created. A deletion strain was used to examine the influence of oxacillin in combination with vanillin on biofilm resistance and cell survival was determined. Utilizing real-time qPCR, confocal laser scanning microscopy (CLSM), and crystal violet staining analyses, we found that the RNAIII-independent controlled phenol soluble modulins (PSMs) and RNAIII effector molecule have a significant role in biofilm resistance to antibiotics and phenolic compounds, and it protects the integrity of biofilms. Moreover, a combination of antibiotic and antimicrobial agents can induce methicillin-resistant S. epidermidis biofilm formation and can lead to exceedingly difficult medical treatment. |
