High Blood Concentration of Leukocyte-Derived Extracellular Vesicles Is Predictive of Favorable Clinical Outcomes in Patients with Pancreatic Cancer: Results from a Multicenter Prospective Study.

Autor:	Brocco D; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., De Bellis D; Department of Medicine and Aging Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.; Center for Advanced Studies and Technology (C.A.S.T.), University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Di Marino P; Clinical Oncology Unit, S.S. Annunziata Hospital, 66100 Chieti, Italy., Simeone P; Department of Medicine and Aging Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.; Center for Advanced Studies and Technology (C.A.S.T.), University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Grassadonia A; Department of Innovative Technologies in Medicine and Dentistry, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., De Tursi M; Department of Innovative Technologies in Medicine and Dentistry, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Grottola T; Surgical Oncology Unit, Casa di Cura Pierangeli, 65124 Pescara, Italy., Di Mola FF; Surgical Oncology Unit, Casa di Cura Pierangeli, 65124 Pescara, Italy., Di Gregorio P; Unit of Transfusion Medicine and Hematology, S.S. Annunziata Hospital, 66100 Chieti, Italy., Zappacosta B; Anatomical Pathology Unit, Casa di Cura Pierangeli, 65124 Pescara, Italy., Angelone A; Anatomical Pathology Unit, Casa di Cura Pierangeli, 65124 Pescara, Italy., Lellis L; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Veschi S; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Florio R; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., De Fabritiis S; Department of Medicine and Aging Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.; Center for Advanced Studies and Technology (C.A.S.T.), University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Verginelli F; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Marchisio M; Department of Medicine and Aging Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.; Center for Advanced Studies and Technology (C.A.S.T.), University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Caporale M; Medical Oncology Unit, S. Spirito Hospital, 65124 Pescara, Italy., Luisi D; Medical Oncology Unit, S. Spirito Hospital, 65124 Pescara, Italy., Di Sebastiano P; Surgical Oncology Unit, Casa di Cura Pierangeli, 65124 Pescara, Italy.; Department of Medical, Oral & Biotechnological Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Tinari N; Department of Medical, Oral & Biotechnological Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Cama A; Department of Pharmacy, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Lanuti P; Department of Medicine and Aging Sciences, University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.; Center for Advanced Studies and Technology (C.A.S.T.), University 'G. D'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.
Jazyk:	angličtina
Zdroj:	Cancers [Cancers (Basel)] 2022 Sep 29; Vol. 14 (19). Date of Electronic Publication: 2022 Sep 29.
DOI:	10.3390/cancers14194748
Abstrakt:	Pancreatic cancer (PC) is one of the leading causes of cancer-related death worldwide. Identification of novel tumor biomarkers is highly advocated in PC to optimize personalized treatment algorithms. Blood-circulating extracellular vesicles hold promise for liquid biopsy application in cancer. We used an optimized flow cytometry protocol to study leukocyte-derived EVs (CD45+) and PD-L1+ EVs in blood from 56 pancreatic cancer patients and 48 healthy controls (HCs). Our results show that PC patients presented higher blood levels of total EVs ( p = 0.0003), leukocyte-derived EVs (LEVs) ( p = 0.001) and PD-L1+ EVs ( p = 0.01), as compared with HCs. Interestingly, a blood concentration of LEVs at baseline was independently associated with improved overall survival in patients with borderline resectable or primary unresectable PC (HR = 0.17; 95% CI 0.04-0.79; p = 0.02). Additionally, increased blood-based LEVs were independently correlated with prolonged progression-free survival (HR = 0.10; 95% CI 0.01-0.82; p = 0.03) and significantly associated with higher disease control rate ( p = 0.02) in patients with advanced PC receiving standard chemotherapy. Notably, a strong correlation between a decrease in blood LEVs concentration during chemotherapy and disease control was observed ( p = 0.005). These intriguing findings point to the potential of LEVs as novel blood-based EV biomarkers for improved personalized medicine in patients affected by PC.
Databáze:	MEDLINE
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