Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles.

Autor: Katti P; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Hall AS; Thermo Fisher Scientific, Houston, TX, USA., Parry HA; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Ajayi PT; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Kim Y; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Willingham TB; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Bleck CKE; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Wen H; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Glancy B; National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Brian.glancy@nih.gov.; National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health Bethesda, Bethesda, MD, USA. Brian.glancy@nih.gov.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Oct 13; Vol. 13 (1), pp. 6058. Date of Electronic Publication: 2022 Oct 13.
DOI: 10.1038/s41467-022-33678-y
Abstrakt: Sustained muscle contraction occurs through interactions between actin and myosin filaments within sarcomeres and requires a constant supply of adenosine triphosphate (ATP) from nearby mitochondria. However, it remains unclear how different physical configurations between sarcomeres and mitochondria alter the energetic support for contractile function. Here, we show that sarcomere cross-sectional area (CSA) varies along its length in a cell type-dependent manner where the reduction in Z-disk CSA relative to the sarcomere center is closely coordinated with mitochondrial network configuration in flies, mice, and humans. Further, we find myosin filaments near the sarcomere periphery are curved relative to interior filaments with greater curvature for filaments near mitochondria compared to sarcoplasmic reticulum. Finally, we demonstrate variable myosin filament lattice spacing between filament ends and filament centers in a cell type-dependent manner. These data suggest both sarcomere structure and myofilament interactions are influenced by the location and orientation of mitochondria within muscle cells.
(© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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