Evaluation of the anti-inflammatory effects of selected cannabinoids and terpenes from Cannabis Sativa employing human primary leukocytes.

Autor: Blevins LK; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA., Bach AP; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA., Crawford RB; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA., Zhou J; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA., Henriquez JE; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA., Rizzo MD; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, USA., Sermet S; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA., Khan DMIO; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA., Turner H; Laboratory of Immunology and Signal Transduction, Chaminade University of Honolulu, Honolulu, HI, USA., Small-Howard AL; GbS Global Biopharma, Ottawa, Canada., Kaminski NE; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA; Center for Research on Ingredient Safety, Michigan State University, East Lansing, MI, USA. Electronic address: kamins11@msu.edu.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2022 Dec; Vol. 170, pp. 113458. Date of Electronic Publication: 2022 Oct 10.
DOI: 10.1016/j.fct.2022.113458
Abstrakt: Cannabis is well established as possessing immune modulating activity. The objective of this study was to evaluate the anti-inflammatory properties of selected cannabis-derived terpenes and cannabinoids. Based on their activity in cannabis-chemovar studies, α-pinene, trans-nerolidol, D-limonene, linalool and phytol were the selected terpenes evaluated. The cannabinoid compounds evaluated included cannabidivarin, cannabidiol, cannabinol, cannabichromene, cannabigerol and delta-9-tetrahydrocannabinol. Human PBMC were pretreated with each compound, individually, at concentrations extending from 0.001 to 10 μM and then stimulated with CpG (plasmacytoid dendritic cell), LPS (monocytes), or anti-CD3/CD28 (T cells). Proliferation, activation marker expression, cytokine production and phagocytosis, were quantified. Of the 21 responses assayed for each compound, cannabinoids showed the greatest immune modulating activity compared to their vehicle control. Delta-9-tetrahydrocannabinol possessed the greatest activity affecting 11 immune parameters followed by cannabidivarin, cannabigerol, cannabichromene, cannabinol and cannabidiol. α-Pinene showed the greatest immune modulating activity from the selected group of terpenes, followed by linalool, phytol, trans-nerolidol. Limonene had no effect on any of the parameters tested. Overall, these studies suggest that selected cannabis-derived terpenes displayed minimal immunological activity, while cannabinoids exhibited a broader range of activity. Compounds possessing anti-inflammatory effects may be useful in decreasing inflammation associated with a range of disorders, including neurodegenerative disorders.
Competing Interests: Declaration of competing interest NK and HL serve on the Scientific Advisory Board for GBs Global Bioparma Inc.
(Copyright © 2022. Published by Elsevier Ltd.)
Databáze: MEDLINE
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