Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution.

Autor: Liao C; Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, Iowa, United States of America., Santoscoy MC; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa United States of America., Craft J; Department of Chemical and Biological Engineering, Biological Materials and Processes (BioMAP) NSF REU Program, Iowa State University, Ames, Iowa, United States of America., Anderson C; Department of Chemical and Biological Engineering, Biological Materials and Processes (BioMAP) NSF REU Program, Iowa State University, Ames, Iowa, United States of America., Soupir ML; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, United States of America., Jarboe LR; Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, Iowa, United States of America.; Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Oct 13; Vol. 17 (10), pp. e0276046. Date of Electronic Publication: 2022 Oct 13 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0276046
Abstrakt: Outer membrane protein A (OmpA) is one of the most abundant outer membrane proteins of Gram-negative bacteria and is known to have patterns of sequence variations at certain amino acids-allelic variation-in Escherichia coli. Here we subjected seven exemplar OmpA alleles expressed in a K-12 (MG1655) ΔompA background to further characterization. These alleles were observed to significantly impact cell surface charge (zeta potential), cell surface hydrophobicity, biofilm formation, sensitivity to killing by neutrophil elastase, and specific growth rate at 42°C and in the presence of acetate, demonstrating that OmpA is an attractive target for engineering cell surface properties and industrial phenotypes. It was also observed that cell surface charge and biofilm formation both significantly correlate with cell surface hydrophobicity, a cell property that is increasingly intriguing for bioproduction. While there was poor alignment between the observed experimental values relative to the known sequence variation, differences in hydrophobicity and biofilm formation did correspond to the identity of residue 203 (N vs T), located within the proposed dimerization domain. The relative abundance of the (I, δ) allele was increased in extraintestinal pathogenic E. coli (ExPEC) isolates relative to environmental isolates, with a corresponding decrease in (I, α) alleles in ExPEC relative to environmental isolates. The (I, α) and (I, δ) alleles differ at positions 203 and 251. Variations in distribution were also observed among ExPEC types and phylotypes. Thus, OmpA allelic variation and its influence on OmpA function warrant further investigation.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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