Fluorescence Correlation Spectroscopy and Phase Separation.
| Autor: | Incicco JJ; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA.; Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA., Roy D; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA.; Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA., Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA.; Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA. soranno@wustl.edu.; Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA. soranno@wustl.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2563, pp. 161-198. |
| DOI: | 10.1007/978-1-0716-2663-4_8 |
| Abstrakt: | A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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