Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models.

Autor: Tangwangvivat R; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.; Division of Communicable Diseases, Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand., Chaiyawong S; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Nonthabenjawan N; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Charoenkul K; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Janethanakit T; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Udom K; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Kesdangsakonwut S; Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Tantilertcharoen R; Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Thontiravong A; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand.; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Amonsin A; Center of Excellences for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, 10330, Bangkok, Thailand. Alongkorn.A@chula.ac.th.; Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. Alongkorn.A@chula.ac.th.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2022 Oct 12; Vol. 19 (1), pp. 162. Date of Electronic Publication: 2022 Oct 12.
DOI: 10.1186/s12985-022-01888-x
Abstrakt: Background: Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important.
Methods: Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10 6 EID 50 per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 10 6 EID 50 per ml of CIV-H3N2.
Results: For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1-2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed.
Conclusion: The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model.
(© 2022. The Author(s).)
Databáze: MEDLINE
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