Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex.

Autor:	Ravi AS; Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA., Zeng M; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China., Chen X; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China., Sandoval G; Department of Anatomy and Neurobiology, University of California at Irvine, Irvine, CA, USA; Center for the Neurobiology of Learning and Memory, University of California at Irvine, Irvine, CA, USA., Diaz-Alonso J; Department of Anatomy and Neurobiology, University of California at Irvine, Irvine, CA, USA; Center for the Neurobiology of Learning and Memory, University of California at Irvine, Irvine, CA, USA. Electronic address: jdiazalo@uci.edu., Zhang M; Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Greater Bay Biomedical Innocenter, Shenzhen Bay Laboratory, Shenzhen 518036, China; School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China., Nicoll RA; Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA. Electronic address: roger.nicoll@ucsf.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2022 Oct 11; Vol. 41 (2), pp. 111483.
DOI:	10.1016/j.celrep.2022.111483
Abstrakt:	The critical role of AMPA receptor (AMPAR) trafficking in long-term potentiation (LTP) of excitatory synaptic transmission is now well established, but the underlying molecular mechanism is still uncertain. Recent research suggests that PSD-95 captures AMPARs via an interaction with the AMPAR auxiliary subunits-transmembrane AMPAR regulatory proteins (TARPs). To determine if such interaction is a core minimal component of the AMPAR trafficking and LTP mechanism, we engineered artificial binding partners, which individually were biochemically and functionally dead but which, when expressed together, rescue binding and both basal synaptic transmission and LTP. These findings establish the TARP/PSD-95 complex as an essential interaction underlying AMPAR trafficking and LTP. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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