The centrosomal protein 83 (CEP83) regulates human pluripotent stem cell differentiation toward the kidney lineage.

Autor:	Mansour F; Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Molecular and Translational Kidney Research, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt., Hinze C; Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Molecular and Translational Kidney Research, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.; Berlin Institute of Health, Berlin, Germany.; Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany., Telugu NS; Berlin Institute of Health, Berlin, Germany.; Technology Platform Pluripotent Stem Cells, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany., Kresoja J; Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, United States., Shaheed IB; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt., Mosimann C; Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, United States., Diecke S; Berlin Institute of Health, Berlin, Germany.; Technology Platform Pluripotent Stem Cells, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany., Schmidt-Ott KM; Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Molecular and Translational Kidney Research, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.; Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2022 Oct 12; Vol. 11. Date of Electronic Publication: 2022 Oct 12.
DOI:	10.7554/eLife.80165
Abstrakt:	During embryonic development, the mesoderm undergoes patterning into diverse lineages including axial, paraxial, and lateral plate mesoderm (LPM). Within the LPM, the so-called intermediate mesoderm (IM) forms kidney and urogenital tract progenitor cells, while the remaining LPM forms cardiovascular, hematopoietic, mesothelial, and additional progenitor cells. The signals that regulate these early lineage decisions are incompletely understood. Here, we found that the centrosomal protein 83 (CEP83), a centriolar component necessary for primary cilia formation and mutated in pediatric kidney disease, influences the differentiation of human-induced pluripotent stem cells (hiPSCs) toward IM. We induced inactivating deletions of CEP83 in hiPSCs and applied a 7-day in vitro protocol of IM kidney progenitor differentiation, based on timed application of WNT and FGF agonists. We characterized induced mesodermal cell populations using single-cell and bulk transcriptomics and tested their ability to form kidney structures in subsequent organoid culture. While hiPSCs with homozygous CEP83 inactivation were normal regarding morphology and transcriptome, their induced differentiation into IM progenitor cells was perturbed. Mesodermal cells induced after 7 days of monolayer culture of CEP83 -deficient hiPCS exhibited absent or elongated primary cilia, displayed decreased expression of critical IM genes ( PAX8 , EYA1 , HOXB7 ), and an aberrant induction of LPM markers (e.g. FOXF1 , FOXF2 , FENDRR , HAND1 , HAND2 ). Upon subsequent organoid culture, wildtype cells differentiated to form kidney tubules and glomerular-like structures, whereas CEP83 -deficient cells failed to generate kidney cell types, instead upregulating cardiomyocyte, vascular, and more general LPM progenitor markers. Our data suggest that CEP83 regulates the balance of IM and LPM formation from human pluripotent stem cells, identifying a potential link between centriolar or ciliary function and mesodermal lineage induction. Competing Interests: FM, CH, NT, JK, IS, CM, SD, KS No competing interests declared (© 2022, Mansour et al.)
Databáze:	MEDLINE
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