Prevalence and Seasonality of Clostridiodes difficile over 12 Years at a Tertiary Hospital in Brazil.

Autor: Maestri AC; Laboratório de Bacteriologia, Complexo Hospital de Clínicas da Universidade Federal do Paraná-Rua Padre Camargo, 280-Alto da Glória, CEP: 80.062-240, Curitiba, Paraná, Brazil.; Programa de Pós-graduação em Medicina Interna e Ciências da Saúde, Universidade Federal do Paraná-Rua General Carneiro, 181-Alto da Glória, CEP-80060-900, Curitiba, Paraná, Brazil., Ando PK; Curso de Medicina, Universidade Federal do Paraná-Rua Padre Camargo, 280-Alto da Glória, CEP: 80.062-240, Curitiba, Paraná, Brazil., Sarot G; Curso de Medicina, Universidade Federal do Paraná-Rua Padre Camargo, 280-Alto da Glória, CEP: 80.062-240, Curitiba, Paraná, Brazil., de Castilho F; Programa de Pós-graduação em Microbiologia, Parasitologia e Patologia, Universidade Federal do Paraná, Centro Politécnico, Av. Cel. Francisco H. dos Santos, 100-Jardim das Américas, CEP-81531-980, Curitiba, Paraná, Brazil., Raboni SM; Programa de Pós-graduação em Medicina Interna e Ciências da Saúde, Universidade Federal do Paraná-Rua General Carneiro, 181-Alto da Glória, CEP-80060-900, Curitiba, Paraná, Brazil.; Departamento de Infectologia, Complexo Hospital de Clínicas da Universidade Federal do Paraná, Rua General Carneiro, 181-Alto da Glória, CEP-80060-900, Curitiba, Paraná, Brazil., Nogueira KS; Laboratório de Bacteriologia, Complexo Hospital de Clínicas da Universidade Federal do Paraná-Rua Padre Camargo, 280-Alto da Glória, CEP: 80.062-240, Curitiba, Paraná, Brazil. keite.nogueira@hc.ufpr.br.; Programa de Pós-graduação em Microbiologia, Parasitologia e Patologia, Universidade Federal do Paraná, Centro Politécnico, Av. Cel. Francisco H. dos Santos, 100-Jardim das Américas, CEP-81531-980, Curitiba, Paraná, Brazil. keite.nogueira@hc.ufpr.br.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2022 Oct 11; Vol. 79 (12), pp. 354. Date of Electronic Publication: 2022 Oct 11.
DOI: 10.1007/s00284-022-03062-6
Abstrakt: Clostridioides difficile is the main pathogen responsible for antibiotic-associated diarrhea in adults. Besides its challenging diagnosis, C. difficile infection (CDI) causes substantial morbidity and mortality. Commercially, there are assays with different targets and performances in sensitivity and specificity. The objectives of this study were to: (1) evaluate the prevalence and seasonal variability of CDI rates at a tertiary hospital in southern Brazil over 12 years and (2) determine the impact of using a two-step algorithm test in the laboratory diagnosis. Between January 2007 and May 2019, fecal samples from 2275 patients were analyzed in a cross-sectional study. Four commercial tests were adopted for the diagnosis of CDI, the immunochromatographic test for toxin A from 2007 to 2010; the enzyme-linked immunosorbent assay method for toxins A and B from 2011 to March 2017; and the rapid enzyme immunoassay (EIA) for GDH and toxins A and B, associated with a Polymerase Chain Reaction (PCR) for the toxin B gene from June 2017 to 2019. The annual prevalence was 8.7% from 2007 to March 2017, increasing between June 2017 and 2019 to 14.7% when the C. diff Quik Chek Complete + GeneXpert C. difficile (two-step algorithm) test was adopted. The number of samples (691) and percentage of CDI cases (10.5%) were higher in winter, but the difference has no statistical significance (P > 0.05). An accurate diagnosis and adequate knowledge of the local seasonality of CDI allow the effective implementation of prevention and control strategies for nosocomial CDI, in addition to effective treatment for patients.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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