First generation of multifunctional peptides derived from latarcin-3a from Lachesana tarabaevi spider toxin.

Autor:	de Moraes LFRN; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.; Selkis Biotech, Startup, Laboratório de Artrópodes Peçonhentos, Campo Grande, MS, Brazil., Silva PSE; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Pereira TCPL; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Almeida Rodrigues TA; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Farias Frihling BE; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.; Selkis Biotech, Startup, Laboratório de Artrópodes Peçonhentos, Campo Grande, MS, Brazil., da Costa RA; Programa de Pós-Graduação em Ciências Genômica e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil., Torquato HFV; Programa de Pós-Graduação em Biotecnologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil., Lima CS; Programa de Pós-Graduação em Biotecnologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil., Paredes-Gamero EJ; Programa de Pós-Graduação em Biotecnologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil., Migliolo L; S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.; Selkis Biotech, Startup, Laboratório de Artrópodes Peçonhentos, Campo Grande, MS, Brazil.; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2022 Sep 21; Vol. 13, pp. 965621. Date of Electronic Publication: 2022 Sep 21 (Print Publication: 2022).
DOI:	10.3389/fmicb.2022.965621
Abstrakt:	The need for discovering new compounds that can act selectively on pathogens is becoming increasingly evident, given the number of deaths worldwide due to bacterial infections or tumor cells. New multifunctional biotechnological tools are being sought, including compounds present in spider venoms, which have high biotechnological potential. The present work aims to perform the rational design and functional evaluation of synthetic peptides derived from Lachesana tarabaevi spider toxin, known as latarcin-3a. The antimicrobial activity was tested against Gram-positive and -negative bacteria, with minimum inhibitory concentrations (MIC) between 4 and 128 μg.ml -1 . Anti-biofilm tests were then performed to obtain MICs, where the peptides demonstrated activity from 4 to 128 μg.ml -1 . In vitro cell cytotoxicity assays were carried out from tumor cell lines, lineages C1498, Kasumi-1, K-562, Jurkat, MOLT4, and Raji. Erythrocyte integrity was evaluated in the presence of synthetic peptides analog, which did not promote hemolysis at 128 μg.ml -1 . The peptide that showed the best antibacterial activity was Lt-MAP3 and the best antitumor was Lt-MAP2. In conclusion, rational design of multifunctional antimicrobial peptides may be promising alternative tools in the treatment of emerging diseases such as bacterial infections and tumor cells. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 de Moraes, Silva, Pereira, Almeida Rodrigues, Farias Frihling, da Costa, Torquato, Lima, Paredes-Gamero and Migliolo.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu