The early response of oil palm ( Elaeis guineensis Jacq.) plants to water deprivation: Expression analysis of miRNAs and their putative target genes, and similarities with the response to salinity stress.

Autor:	Salgado FF; Graduate Program of Plant Biotechnology, Federal University of Lavras, Lavras, MG, Brazil., da Silva TLC; Graduate Program of Plant Biotechnology, Federal University of Lavras, Lavras, MG, Brazil., Vieira LR; Graduate Program of Plant Biotechnology, Federal University of Lavras, Lavras, MG, Brazil., Silva VNB; Graduate Program of Plant Biotechnology, Federal University of Lavras, Lavras, MG, Brazil., Leão AP; The Brazilian Agricultural Research Corporation, Embrapa Agroenergy, Brasília, DF, Brazil., Costa MMDC; The Brazilian Agricultural Research Corporation, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., Togawa RC; The Brazilian Agricultural Research Corporation, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., de Sousa CAF; The Brazilian Agricultural Research Corporation, Embrapa Mid-North, Teresina, PI, Brazil., Grynberg P; The Brazilian Agricultural Research Corporation, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., Souza MT Jr; Graduate Program of Plant Biotechnology, Federal University of Lavras, Lavras, MG, Brazil.; The Brazilian Agricultural Research Corporation, Embrapa Agroenergy, Brasília, DF, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Sep 23; Vol. 13, pp. 970113. Date of Electronic Publication: 2022 Sep 23 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.970113
Abstrakt:	Oil palm ( Elaeis guineensis Jacq.) is a oilseed crop of great economic importance drastically affected by abiotic stresses. MicroRNAs (miRNAs) play crucial roles in transcription and post-transcription regulation of gene expression, being essential molecules in the response of plants to abiotic stress. To better understand the molecular mechanisms behind the response of young oil palm plants to drought stress, this study reports on the prediction and characterization of miRNAs and their putative target genes in the apical leaf of plants subjected to 14 days of water deprivation. Then, the data from this study were compared to the data from a similar study that focused on salinity stress. Both, the drought-and salt-responsive miRNAs and their putative target genes underwent correlation analysis to identify similarities and dissimilarities among them. Among the 81 identified miRNAs, 29 are specific for oil palm, including two (egu-miR28ds and egu-miR29ds) new ones - described for the first time. As for the expression profile, 62 miRNAs were significantly differentially expressed under drought stress, being five up-regulated (miR396e, miR159b, miR529b, egu-miR19sds, and egu-miR29ds) and 57 down-regulated. Transcription factors, such as MYBs, HOXs, and NF-Ys, were predicted as putative miRNA-target genes in oil palm under water deprivation; making them the most predominant group of such genes. Finally, the correlation analysis study revealed a group of putative target genes with similar behavior under salt and drought stresses. Those genes that are upregulated by these two abiotic stresses encode lncRNAs and proteins linked to stress tolerance, stress memory, modulation of ROS signaling, and defense response regulation to abiotic and biotic stresses. In summary, this study provides molecular evidence for the possible involvement of miRNAs in the drought stress response in oil palm. Besides, it shows that, at the molecular level, there are many similarities in the response of young oil palm plants to these two abiotic stresses. Competing Interests: AL, MC, RT, CS, PG, and MS were employed by The Brazilian Agricultural Research Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Salgado, da Silva, Vieira, Silva, Leão, Costa, Togawa, de Sousa, Grynberg and Souza.)
Databáze:	MEDLINE
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