Neuroprotective Effects of the Ethanolic Leaf Extract of Crassocephalum crepidioides (Asteracaeae) on Diazepam-Induced Amnesia in Mice.

Autor: Galba Jean B; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Alice Irène F; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Albert Donatien A; Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaoundé, Cameroon., Hervé-Hervé NA; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Bertrand BM; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Nanou Gael AD; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Merline NY; Department of Biological Science, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon., Franklik ZG; Department of Psychology, Faculty of Arts, Letters and Social Science, University of Yaoundé I, P.O. Box 7011, Yaoundé, Cameroon., Bertrand DA; Department of Animal Biology Faculty of Science University of Douala, P.O. Box 24157, Douala, Cameroon., Théophile D; Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaoundé, Cameroon.
Jazyk: angličtina
Zdroj: Advances in pharmacological and pharmaceutical sciences [Adv Pharmacol Pharm Sci] 2022 Sep 28; Vol. 2022, pp. 1919469. Date of Electronic Publication: 2022 Sep 28 (Print Publication: 2022).
DOI: 10.1155/2022/1919469
Abstrakt: This study aimed to evaluate the neuroprotective effects of the ethanolic leaf extract of Crassocephalum crepidioides ( Cc ) on diazepam-induced amnesia in mice. Thirty mice distributed into six groups of five mice each were used. The normal control and negative control groups received 2% ethanol per os, the positive control group received piracetam (150 mg/kg, p.o ), and three experimental groups were treated with three doses of ethanolic leaf extract of Cc (100, 200, and 400 mg/kg, p.o ). All groups except the normal control group were co-treated with diazepam (3 mg/kg, i.p ) daily for 14 days. The memory effects were evaluated using the Radial Arm Maze (RAM) and the Novel Object Recognition (NOR) tests, while the anti-depressive effects were evaluated using the tail suspension test. All animals were sacrificed at the end of the study. Hippocampi, isolated from the right hemisphere, were used to prepare a homogenate for the determination of oxidative stress biomarkers. The ethanolic leaf extract of cc significantly ( p < 0.001) decreased the number of working and reference memory errors in the RAM test and induced a significant ( p < 0.01) increase in the time spent exploring the novel object in the NOR test. The extract also induced a significant ( p < 0.001) increase in the mobility time in tail suspension. Moreover, compared to the negative control group, the extract significantly ( p < 0.01) increased superoxide dismutase activity and significantly ( p < 0.01) decreased malondialdehyde levels. The histopathological analysis of hippocampi showed that the cc extract increased cell density when compared with the negative control. These results suggest that the ethanolic left extract of cc could have neuroprotective properties, which could be attributed to its antioxidant properties.
Competing Interests: The authors declare that they have no conflicts of interest related to the publication of this study.
(Copyright © 2022 Beppe Galba Jean et al.)
Databáze: MEDLINE
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