In vitro and in vivo anthelmintic properties of Caesalpinia coriaria fruits against Haemonchus contortus.

Autor: García-Hernández C; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carretera Federal Toluca-Tejupilco, Temascaltepec, C.P. 51300, Estado de, México., Rojo-Rubio R; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carretera Federal Toluca-Tejupilco, Temascaltepec, C.P. 51300, Estado de, México., Gives PM; Unidad de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Carretera Federal Cuernavaca-Cuautla, No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos/A.P, 206-CIVAC, México., González-Cortazar M; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, C.P. 62790, Xochitepec, Morelos, México., Zamilpa A; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, C.P. 62790, Xochitepec, Morelos, México., Mondragón-Ancelmo J; Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, km 67.5. Carretera Federal Toluca-Tejupilco, Temascaltepec, C.P. 51300, Estado de, México., Villa-Mancera A; Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco, Puebla, México., Olivares-Pérez J; Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciudad Altamirano, Guerrero, México., Tapia-Maruri D; Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, Km. 6, colonia San Isidro, C.P. 62731, Yautepec, Morelos, México., Olmedo-Juárez A; Unidad de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Carretera Federal Cuernavaca-Cuautla, No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos/A.P, 206-CIVAC, México. Electronic address: aolmedoj@gmail.com.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2022 Nov; Vol. 242, pp. 108401. Date of Electronic Publication: 2022 Oct 06.
DOI: 10.1016/j.exppara.2022.108401
Abstrakt: The purpose of the present study was to assay the in vitro and in vivo anthelmintic activity (AA) of Caesalpinia coriaria (Cc) mature fruits against the nematode Haemonchus contortus (Hc). The Hc infective larvae were used to assess the in vitro AA through larval mortality assay. The exposure of larvae to the different treatments was performed in 96-well microtitration plates. The treatments were as follows: hydroalcoholic extract (HA-E, at 25-100 mg/mL), aqueous fraction (Aq-F, at 12.5-50 mg/mL), organic fraction (EtOAc-F at 12.5-50 mg/mL), compounds (1, methyl gallate and 2, gallic acid at 1.25-10 mg/mL), positive control (ivermectin at 5 mg/mL) and two negative controls (distilled water and 4% methanol). After exposure, dead and live larvae were quantified and results were compared to their controls. The in vivo assay was carried out by a faecal egg count reduction test (FECRT); artificially infected goat kids (F1: Boer x Nubia) were treated with Cc ground dried fruits to assess the AA. The treatments were established as follows: G1-untreated goats (negative control), G2-goats dewormed with ivermectin (positive control), G3-goats fed with Cc mature fruits (10% of their diet). Results in both in vitro and in vivo assays were analysed using an ANOVA through random design, applying a general linear model and mixed models. The in vitro results showed an evident larvicidal effect of the HA-E, EtOAc-F from Cc, indicating that the compound responsible for the AA was gallic acid. The results of the in vivo study corroborated the anthelmintic properties of Cc, reaching 78.6% reduction in the elimination of Hc eggs per gram of faeces. This plant represents a potential natural anthelmintic for the control of haemonchosis in goats under grazing conditions. Future studies should standardise the Cc extract or dried fruits for use in the management of nematodiasis in goat herds.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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