Taenia hydatigena larvae vesicular concentrates increase Anti-OVA IgG and the production of some cytokines in rats.

Autor: Cuenca-Verde C; Programa de Maestría y Doctorado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México, 04510, Ciudad Universitaria, Ciudad de México, Mexico; Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Km. 2.5 Carretera Cuautitlán-Teoloyucan S/N, 54714, Cuautitlán Izcalli, MEX, Mexico., Muñoz-Guzmán MA; Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Km. 2.5 Carretera Cuautitlán-Teoloyucan S/N, 54714, Cuautitlán Izcalli, MEX, Mexico., Del Rio-Araiza VH; Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, AP 70228, Ciudad de México, 04510, Mexico., Valdivia-Anda G; Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Km. 2.5 Carretera Cuautitlán-Teoloyucan S/N, 54714, Cuautitlán Izcalli, MEX, Mexico., Olguín JE; Laboratorio Nacional en Salud, Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, y Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios Número 1, 54090, Tlalnepantla de Baz, Mexico., Terrazas LI; Laboratorio Nacional en Salud, Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, y Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios Número 1, 54090, Tlalnepantla de Baz, Mexico., Morales-Montor J; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de México, 04510, Mexico., Alba-Hurtado F; Departamento de Ciencias Biológicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Km. 2.5 Carretera Cuautitlán-Teoloyucan S/N, 54714, Cuautitlán Izcalli, MEX, Mexico. Electronic address: fealba@hotmail.com.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2022 Nov; Vol. 242, pp. 108400. Date of Electronic Publication: 2022 Oct 05.
DOI: 10.1016/j.exppara.2022.108400
Abstrakt: The effects of administration of four different fractions of T. hydatigena larvae vesicular concentrate (ThLVC) prior to immunization with ovalbumin (OVA) in rats on different parameters of the immune response were evaluated. The amount of anti-OVA IgG by ELISA, amount of blood eosinophils (BE), percentage of cell subpopulations by flow cytometry (CD3+/CD4+, CD3+/CD8+, CD3-/CD45RA+, and CD11b/c+), and production of serum cytokines by bead-based immunoassays (IL-2, IL-4, INFγ, IL-5, TNFα, GM-CSF, IL-17F, IL-17A IL-13, IL-22, and IL-6) were measured. Rats receiving total-ThLVC (p ≤ 0.05) and fraction ThLVC30-100 kDa (p < 0.001) prior to OVA administration produced higher amounts of anti-OVA IgG than rats receiving OVA alone. Rats that were only administered with OVA showed a strong increase in BE that was significantly correlated (r = 0.72, p < 0.001) with an increase in IL-5 in the blood. However, rats that received any of the ThLVC fractions prior to administration of OVA did not show these increases. In general, administration of ThLVC30-100 kDa prior to administration of OVA increased (p < 0.05) the percentage of B, CD4, and CD8 lymphocytes in the spleen and mesenteric lymph nodes. Rats that received ThLVC total fraction and OVA showed an increase (p < 0.05) in IL-2, IL17F, and IL22. The results of this study show that total-ThLVC and ThLVC30-100 kDa modify the immune response of rats in differentiated ways. Our observations suggests that both fractions of ThLVC have the potential to be used as adjuvants.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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