3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.

Autor: Barin N; Department of Precision and Microsystems Engineering, Delft University of Technology, Mekelweg 2, Delft, 2628 CD, The Netherlands.; Department of Neurology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., Balcioglu HE; Department of Medical Oncology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., de Heer I; Department of Neurology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., de Wit M; Department of Neurology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., Lamfers MLM; Department of Neurosurgery, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., van Royen ME; Department of Pathology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., French PJ; Department of Neurology, Erasmus MC Cancer Institute, University Medical Center, Doctor Molewaterplein 40, Rotterdam, 3015 GD, The Netherlands., Accardo A; Department of Precision and Microsystems Engineering, Delft University of Technology, Mekelweg 2, Delft, 2628 CD, The Netherlands.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2022 Dec; Vol. 18 (49), pp. e2204485. Date of Electronic Publication: 2022 Oct 07.
DOI: 10.1002/smll.202204485
Abstrakt: A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.
(© 2022 The Authors. Small published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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