Value of immunohistochemistry in crushed areas of pulmonary neuroendocrine carcinoma.

Autor: Kuçuk H; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France., Laville D; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France., Dal-Col P; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France., Yvorel V; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France; Department of Molecular Biology of Solid Tumors, University Hospital of Saint Etienne, Saint Etienne, France., Sulaiman A; Department of Thoracic Surgery, University Hospital of Saint Etienne, Saint Etienne, France., Bayle-Bleuez S; Department of Pneumology, University Hospital of Saint Etienne, Saint Etienne, France., Cosmo P; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France., Vergnon JM; Department of Pneumology, University Hospital of Saint Etienne, Saint Etienne, France., Tiffet O; Department of Thoracic Surgery, University Hospital of Saint Etienne, Saint Etienne, France., Desage AL; Department of Pneumology, University Hospital of Saint Etienne, Saint Etienne, France., Forest F; Department of Pathology, University Hospital of Saint Etienne, Saint Etienne, France; Department of Molecular Biology of Solid Tumors, University Hospital of Saint Etienne, Saint Etienne, France. Electronic address: f.forest@univ-st-etienne.fr.
Jazyk: angličtina
Zdroj: Experimental and molecular pathology [Exp Mol Pathol] 2022 Oct; Vol. 128, pp. 104836. Date of Electronic Publication: 2022 Oct 04.
DOI: 10.1016/j.yexmp.2022.104836
Abstrakt: Immunohistochemical demonstration of neuroendocrine differentiation is often performed in routine diagnostic practice for lung neuroendocrine carcinoma. However, these carcinomas are often crushed, especially on small specimens. The value of immunohistochemistry on crushed areas is not known. We aimed to assess the value of immunohistochemical markers in crushed areas. We performed a retrospective study of 299 patients with a diagnosis of pulmonary neuroendocrine carcinoma. We showed that the markers TTF-1, synaptophysin, chromogranin A, CD56, and INSM1 were more often negative in crushed areas compared with well-preserved areas. The proliferation index with anti-Ki67 was decreased but remained on average around 90%. For all markers, the percentage of labeled cells was lower than in the preserved areas. Finally, we show that cases without labeling in the crushed areas and maintained labeling in the non-crushed areas have a lower percentage of labeling than cases without this labeling mismatch. Finally, there were no false positives of these stains. Neuroendocrine markers are valid in crushed areas when positive. However, the percentage of labeled cells may be lower than on preserved areas and lead to false negatives. Finally, the proliferation index, although decreased, remains close to that on preserved areas.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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