Transmission Electron Microscopy: A Method for Studying the Adrenal Chromaffin Cells.

Autor:	Capaldo A; Department of Biology, University of Naples Federico II, Naples, Italy. anna.capaldo@unina.it.; Center for Studies on Bioinspired Agro-environmental Technology (BAT Center), Portici, Italy. anna.capaldo@unina.it., De Falco M; Department of Biology, University of Naples Federico II, Naples, Italy.; Center for Studies on Bioinspired Agro-environmental Technology (BAT Center), Portici, Italy.; National Institute of Biostructures and Biosystems (INBB), Rome, Italy., Rosati L; Department of Biology, University of Naples Federico II, Naples, Italy.; Center for Studies on Bioinspired Agro-environmental Technology (BAT Center), Portici, Italy., Laforgia V; Department of Biology, University of Naples Federico II, Naples, Italy.; National Institute of Biostructures and Biosystems (INBB), Rome, Italy.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2565, pp. 43-55.
DOI:	10.1007/978-1-0716-2671-9_4
Abstrakt:	Transmission electron microscopy and the use of glutaraldehyde-osmium fixation allow to distinguish norepinephrine from epinephrine granules in the adrenochromaffin cells, a difficult distinction with histochemical methods if both types of granules are present in the same cell. Here we describe all the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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