AuNPs-COFs Core-Shell Reversible SERS Nanosensor for Monitoring Intracellular Redox Dynamics.

Autor: Chen ZC; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Xu HB; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Chen HY; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Zhu SC; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Huang WF; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., He Y; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Hafez ME; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.; Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt., Qian RC; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China., Li DW; Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2022 Oct 18; Vol. 94 (41), pp. 14280-14289. Date of Electronic Publication: 2022 Oct 06.
DOI: 10.1021/acs.analchem.2c02814
Abstrakt: The redox homeostasis in living cells is greatly crucial for maintaining the redox biological function, whereas accurate and dynamic detection of intracellular redox states still remains challenging. Herein, a reversible surface-enhanced Raman scattering (SERS) nanosensor based on covalent organic frameworks (COFs) was prepared to dynamically monitor the redox processes in living cells. The nanosensor was fabricated by modifying the redox-responsive Raman reporter molecule, 2-Mercaptobenzoquione (2-MBQ), on the surface of gold nanoparticles (AuNPs), followed by the in situ coating of COFs shell. 2-MBQ molecules can repeatedly and quickly undergo reduction and oxidation when successively treated with ascorbic acid (AA) and hypochlorite (ClO - ) (as models of reductive and oxidative species, respectively), which resulted in the reciprocating changes of SERS spectra at 900 cm -1 . The construction of the COFs shell provided the nanosensor with great stability and anti-interference capability, thus reliably visualizing the dynamics of intracellular redox species like AA and ClO - by SERS nanosensor. Taken together, the proposed SERS strategy opens up the prospects to investigate the signal transduction pathways and pathological processes related with redox dynamics.
Databáze: MEDLINE