Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model.
| Autor: | Dwyer-Nield L; University of Colorado School of Pharmacy, Aurora, CO 80045, USA., Keith RL; Rocky Mountain Regional VA Medical Center, Aurora, CO 80045, USA; University of Colorado School of Medicine, Aurora, CO 80045, USA., Tennis MA; University of Colorado School of Medicine, Aurora, CO 80045, USA. Electronic address: meredith.tennis@cuanschutz.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2022 Dec 16; Vol. 3 (4), pp. 101750. Date of Electronic Publication: 2022 Oct 04. |
| DOI: | 10.1016/j.xpro.2022.101750 |
| Abstrakt: | Mouse iloprost lung cancer chemoprevention studies typically use oral delivery. Here, we present a protocol for intranasal iloprost delivery within a urethane lung adenocarcinoma mouse model. We detail steps for intraperitoneal urethane injection in mice, followed by nine-week monitoring, intranasal iloprost treatment, and lungs harvesting for analysis. This iloprost delivery approach parallels an ongoing phase II clinical trial of inhaled iloprost for lung cancer chemoprevention. This protocol diversifies options for chemoprevention studies and offers a relevant and translatable model. For complete details on the use and execution of this protocol, please refer to Sompel et al. (2022). Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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