Magnetic fields as inducer of glutathione and peroxidase production by Saccharomyces cerevisiae.

Autor: Machado BR; Laboratory of Biotechnology, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil., Silva PGP; Laboratory of Biotechnology, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil., Garda-Buffon J; Laboratory of Mycotoxin and Food Science, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil., Santos LO; Laboratory of Biotechnology, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil. santoslucielen@gmail.com.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2022 Dec; Vol. 53 (4), pp. 1881-1891. Date of Electronic Publication: 2022 Oct 06.
DOI: 10.1007/s42770-022-00836-9
Abstrakt: Glutathione (GSH) and peroxidase (POD) are biomolecules of interest in the global market; thus, it is desirable to seek ways to increase their production. Magnetic field (MF) application is one of the technologies used in cultivation that has shown promising results to increase bioproducts. Therefore, this study aimed at evaluating the influence of MFs on GSH and POD production by Saccharomyces cerevisiae ATCC 7754. Different periods of MF application (35 mT) were evaluated over 72 h. The highest GSH production was reached in 48 h of cultivation in assays MF 0-24 (155.32 ± 9.12 mg L -1 ) and MF 0-72 (149.27 ± 3.62 mg L -1 ), which showed an increase of 121.9 % and 113 %, respectively, by comparison with the control without any MF application. The highest POD activity was achieved when MFs were applied throughout the culture (36.31 U mg -1 ) and POD productivity of 0.72 U mg -1  h -1 . MF application throughout cultivation proved to be a promising strategy since all responses increased, i.e., GSH concentration, GSH productivity, POD activity, and POD productivity increased 113.7 %, 113 %, 20.4 %, and 28.6 %, respectively. This study is one of the first to consider MFs as a viable and low-cost alternative to produce GSH and POD in bioprocesses.
(© 2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
Externí odkaz: