| Autor: |
Michaelis VK; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; Department of Chemistry, University of Alberta, Edmonton T6G 2G2, Alberta, Canada., Keeler EG; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; New York Structural Biology Center, 89 Convent Avenue, New York 10027, New York, United States., Bahri S; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht 3584CH, The Netherlands., Ong TC; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles 90095, California, United States., Daviso E; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; Department of Scientific Support and Applications Development, Covaris LLC, Woburn 01801, Massachusetts, United States., Colvin MT; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States.; Ortho Clinical Diagnostics, 130 Indigo Creek Dr, Rochester 14626, New York, United States., Griffin RG; Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, Massachusetts, United States. |
| Abstrakt: |
The sensitivity enhancements available from dynamic nuclear polarization (DNP) are rapidly reshaping the research landscape and expanding the field of nuclear magnetic resonance (NMR) spectroscopy as a tool for solving complex chemical and structural problems. The past decade has seen considerable advances in this burgeoning method, while efforts to further improve its capabilities continue along many avenues. In this report, we examine the influence of static magnetic field strength and temperature on the reported 1 H DNP enhancements from three conventional organic biradicals: TOTAPOL, AMUPol, and SPIROPOL. In contrast to the conventional wisdom, our findings show that at liquid nitrogen temperatures and 700 MHz/460.5 GHz, these three bisnitroxides all provide similar 1 H DNP enhancements, ε ≈ 60. Furthermore, we investigate the influence of temperature, microwave power, magnetic field strength, and protein sample deuteration on the NMR experimental results. |
