Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures.

Autor: Shiea J; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan.; Rapid Screening Research Center for Toxicology and Biomedicine, National Sun Yat-Sen University, Kaohsiung, Taiwan.; Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan., Lin HJ; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Bhat SM; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Lee CY; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Huang MZ; Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan., Ponnusamy VK; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan., Cheng SC; Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan.; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2022 Dec 30; Vol. 36 (24), pp. e9409.
DOI: 10.1002/rcm.9409
Abstrakt: Flame-induced atmospheric pressure chemical ionization (FAPCI) has been used to directly characterize chemical compounds on a glass rod and drug tablet surfaces. In this study, FAPCI was further applied to interface thin layer chromatography (TLC) and mass spectrometry (MS) for mixture analysis.
Methods: A micro-sized oxyacetylene flame was generated using a small concentric tube system. Hot gas flow and primary reactive species from the micro-flame were directed toward a developed TLC gel plate to thermally desorb and ionize analytes on the gel surface. The resulting analyte ions subsequently entered the MS inlet for detection.
Results: A 1-1.5-mm-wide light-brown line was observed on the TLC plate after the desorption FAPCI/MS (DFAPCI/MS) analysis, revealing that the gel surface withstood a high temperature from the impact of the micro-flame. Volatile and semi-volatile chemical compounds, including amine and amide standards, drugs, and aromatherapy oils, were successfully desorbed, ionized, and detected using this TLC/DFAPCI/MS. The limit of detection of TLC-DFAPCI/MS was determined to be 5 ng/spot for dibenzylamine and ethenzamide.
Conclusions: TLC/DFAPCI/MS is one of the simplest TLC-MS interfaces showing the advantages such as low costs and an easy set up. The technique is useful for characterizing thermally stable volatile and semi-volatile compounds in a mixture.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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