A novel bistable device to study mechanosensitive cell responses to instantaneous stretch.

Autor: Choi Y; Department of Mechanical and Process Engineering, ETH Zurich, Zurich 8092, Switzerland., Morlino G; Benevolent AI, W1T 5HD London, UK., Toboso-Navasa A; Benevolent AI, W1T 5HD London, UK., Hopf R; Department of Mechanical and Process Engineering, ETH Zurich, Zurich 8092, Switzerland; Swiss Federal Laboratories for Materials Science and Technology (EMPA), Dübendorf 8600, Switzerland; Senecell AG, Zurich 8057, Switzerland., Pramotton FM; Department of Mechanical and Process Engineering, ETH Zurich, Zurich 8092, Switzerland; Swiss Federal Laboratories for Materials Science and Technology (EMPA), Dübendorf 8600, Switzerland., Bigot A; Sorbonne Université, Inserm, Institut de Myologie, Centre de Recherche en Myologie, F-75013 Paris, France., Taddei A; Benevolent AI, W1T 5HD London, UK., Cesarovic N; Department of Health Sciences and Technology, ETH Zurich, Zurich 8092, Switzerland; Charité - Universitätsmedizin Berlin, Berlin 10117, Germany., Falk V; Department of Health Sciences and Technology, ETH Zurich, Zurich 8092, Switzerland; Charité - Universitätsmedizin Berlin, Berlin 10117, Germany., Mazza E; Department of Mechanical and Process Engineering, ETH Zurich, Zurich 8092, Switzerland; Swiss Federal Laboratories for Materials Science and Technology (EMPA), Dübendorf 8600, Switzerland. Electronic address: mazza@imes.mavt.ethz.ch., Giampietro C; Department of Mechanical and Process Engineering, ETH Zurich, Zurich 8092, Switzerland; Swiss Federal Laboratories for Materials Science and Technology (EMPA), Dübendorf 8600, Switzerland; Senecell AG, Zurich 8057, Switzerland. Electronic address: costanza.giampietro@empa.ch.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2022 Oct; Vol. 141, pp. 213134. Date of Electronic Publication: 2022 Sep 28.
DOI: 10.1016/j.bioadv.2022.213134
Abstrakt: The behavior of cells and tissues in vivo is determined by the integration of multiple biochemical and mechanical signals. Of the mechanical signals, stretch has been studied for decades and shown to contribute to pathophysiological processes. Several different stretch devices have been developed for in vitro investigations of cell stretch. In this work, we describe a new 3D-printed uniaxial stretching device for studying cell response to rapid deformation. The device is a bistable compliant mechanism holding two equilibrium states-an unstretched and stretched configuration-without the need of an external actuator. Furthermore, it allows multiple simultaneous measurements of different levels of stretch on a single substrate and is compatible with standard immunofluorescence imaging of fixed cells as well as live-cell imaging. To demonstrate the effectiveness of the device to stretch cells, a test case using aligned myotubes is presented. Leveraging material area changes associated with deformation of the substrate, changes in nuclei density provided evidence of affine deformation between cells and substrate. Furthermore, intranuclear deformations were also assessed and shown to deform non-affinely. As a proof-of-principle of the use of the device for mechanobiological studies, we uniaxially stretched aligned healthy and dystrophic myotubes that displayed different passive mechanical responses, consistent with previous literature in the field. We also identified a new feature in the mechanoresponse of dystrophic myotubes, which is of potential interest for identifying the diseased cells based on a quick mechanical readout. While some applications of the device for elucidating passive mechanical responses are demonstrated, the simplicity of the device allows it to be potentially used for other modes of deformation with little modifications.
Competing Interests: Declaration of competing interest Authors have no competing interests to declare.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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