Chronic wasting disease prions in mule deer interdigital glands.

Autor: Ness A; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada., Zeng D; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, Alberta, Canada.; Hotchkiss Brain Institute, University of Calgary, Calgary, Canada., Kuznetsova A; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada.; Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, Canada., Otero A; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada., Kim C; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada., Saboraki K; Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada., Lingle S; Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada., Pybus M; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.; Alberta Environment and Parks, Edmonton, Alberta, Canada., Aiken J; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada.; Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, Canada., Gilch S; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, Alberta, Canada.; Hotchkiss Brain Institute, University of Calgary, Calgary, Canada., McKenzie D; Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.; Centre for Prions and Protein Folding Diseases, Edmonton, Alberta, Canada.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Oct 03; Vol. 17 (10), pp. e0275375. Date of Electronic Publication: 2022 Oct 03 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0275375
Abstrakt: Chronic wasting disease (CWD) is a geographically expanding, fatal neurodegenerative disease in cervids. The disease can be transmitted directly (animal-animal) or indirectly via infectious prions shed into the environment. The precise mechanisms of indirect CWD transmission are unclear but known sources of the infectious prions that contaminate the environment include saliva, urine and feces. We have previously identified PrPC expression in deer interdigital glands, sac-like exocrine structures located between the digits of the hooves. In this study, we assayed for CWD prions within the interdigital glands of CWD infected deer to determine if they could serve as a source of prion shedding and potentially contribute to CWD transmission. Immunohistochemical analysis of interdigital glands from a CWD-infected female mule deer identified disease-associated PrPCWD within clusters of infiltrating leukocytes adjacent to sudoriferous and sebaceous glands, and within the acrosyringeal epidermis of a sudoriferous gland tubule. Proteinase K-resistant PrPCWD material was amplified by serial protein misfolding cyclic amplification (sPMCA) from soil retrieved from between the hoof digits of a clinically affected mule deer. Blinded testing of interdigital glands from 11 mule deer by real-time quake-induced conversion (RT-QuIC) accurately identified CWD-infected animals. The data described suggests that interdigital glands may play a role in the dissemination of CWD prions into the environment, warranting future investigation.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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