| Autor: |
Datta R; Department of Botany, Dr. A.P.J. Abdul Kalam Government College, Newtown, West Bengal, India., Mandal K; Plant Biology Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata India., Boro P; Plant Biology Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata India., Sultana A; Department of Botany, J. K. College, Purulia, West Bengal, India., Chattopadhyay S; Plant Biology Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata India. |
| Jazyk: |
angličtina |
| Zdroj: |
Plant signaling & behavior [Plant Signal Behav] 2022 Dec 31; Vol. 17 (1), pp. 2047352. |
| DOI: |
10.1080/15592324.2022.2047352 |
| Abstrakt: |
Glutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infection in Arabidopsis thaliana , wild-type Col-0 and AtECS1 , the transgenic line exhibiting enhanced GSH content, were infected with necrotrophic pathogen Alternaria brassicicola. AtECS1 plants exhibited enhanced resistance as compared to wild-type. MiRNA next-generation sequencing (NGS) was performed to compare the miRNA expression in Col-0 and AtECS 1 leaves. Under control condition, differentially expressed 96 known miRNAs and 17 novel miRNAs viz. ath-miR8167f, ath-miR1886.3, ath-miR3932b-5p, etc. were identified. However, under infected condition, 73 known and 43 novel differentially expressed miRNAs viz. ath-miR5652, ath-miR160b, ath-miR865-5p, etc. were identified. Functional annotation and enrichment analysis revealed that several miRNAs that target defense-related genes like leucine-rich repeat protein kinase, MYB transcription factors, TCP8, etc. were down regulated in the AtECS1 line, which, in turn, relieves the repression of their target gene expression, leading to resistance against infection. Together, the present investigation suggests that GSH plays a decisive role in modulating the miRNA-mediated regulation of defense-related genes during pathogen invasion. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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